何仲秋 刘洋 王艺 王晓琳 解梦凡 闫慧峰

摘要 [目的]分析缺氮條件下烤烟叶片性状变化特征，明确叶片性状与叶绿素含量的关系，为优化烤烟氮肥管理策略提供理论依据。[方法]采用大田试验，以云烟87为材料，设置对照施肥和不施氮肥2个处理，在移栽90 d后比较不同处理叶片性状特征（包括叶片干重、叶面积、比叶重、叶片厚度、叶片密度、叶长、叶宽）和叶绿素含量的差异，分析叶片性状和单位面积叶绿素含量以及单位质量叶绿素含量间的关系。[结果]在缺氮条件下，烤烟中、上部叶的叶片干重、叶面积、比叶重、叶长、叶宽和单位面积叶绿素含量减小，叶片厚度无显著变化。下部叶的叶面积和叶片厚度显著减小。单位叶面积叶绿素含量与比叶重和叶片厚度呈极显著正相关，单位质量叶绿素与叶片厚度呈极显著正相关。[结论]不施氮肥影响了烤烟叶片性状，烤烟通过减小叶面积保持叶片厚度来提高叶绿素含量。

关键词 烤烟;氮;叶片性状;叶绿素含量

中图分类号 S572文献标识码 A

文章编号 0517-6611（2021）10-0149-04

doi：10.3969/j.issn.0517-6611.2021.10.040

开放科学（资源服务）标识码（OSID）：

Effects of Nitrogen Deficiency on Leaf Traits and Chlorophyll Content of Flue-cured Tobacco

HE Zhong-qiu1，LIU Yang2，WANG Yi2et al （1.Tobacco Research Institute of CAAS/Key Laboratory of Tobacco Biology and Processing，Ministry of Agriculture and Rural Affairs，Qingdao，Shandong 266101;2.Linqu Tobacco Branch of Weifang Tobacco Corporation，Linqu，Shandong262600）

Abstract [Objective] To provide theoretical basis for optimizing nitrogen management strategy of flue-cured tobacco，the change characteristics of leaf traits under nitrogen deficiency were analyzed and the relationship between leaf traits and chlorophyll content was established.[Method]In field experiment，Yunyan 87 was used as the material，and two treatments of control fertilization and nitrogen-deficiency fertilization were set up.After 90 days of transplanting，the differences of leaftraits （including leaf dry weight，leaf area，specific leaf weight，leaf thickness，leaf density，leaf length and leaf width） and chlorophyll content of different treatments were compared，the relationship between leaf traits and chlorophyll content per unit area and unit mass of chlorophyll content were analyzed.[Result]Under the condition of nitrogen deficiency，leaf dry weight，leaf area，specific leaf weight，leaf length，leaf width and chlorophyll content per unit area of middle and upper leaves of flue-cured tobacco decreased，but there was no significant change in leaf thickness.The leaf area and thickness of the lower leaves decreased significantly.Chlorophyll content per unit leaf area was significantly positively correlated with specific leaf weight and leaf thickness，and chlorophyll content per unit mass was significantly positively correlated with leaf thickness.[Conclusion]The lack of nitrogen fertilizer affects the traits of flue-cured tobacco leaves.Leaf chlorophyll content was increased by reducing leaf area and maintaining leaf thickness.

Key words Flue-cured tobacco;Nitrogen;Leaf characters;Chlorophyll content

氮素是植物生长必需的元素之一，氮肥的大量使用已经引起越来越严重的环境和生态问题[1]。与主要粮食作物不同，烟草以叶片为收获器官，产量和品质是实现烟草生产最大经济效益的2个决定性因素，而叶片的产量和品质分别受到氮肥用量的正负调控[2]。叶片是烟草进行光合作用的主要器官，烟草产量和品质的形成依赖于烟叶光合作用产生的有机物质[3] 。……