蒙怡 查锦宏 朱蕴兰 邵颖 陈安徽 岳明宇 朱文静 张爱文

摘要 [目的]研究球孢白僵菌定殖對牛蒡苗生长特性的影响。[方法]采用浸种法将球孢白僵菌定殖牛蒡苗，通过测定牛蒡苗的幼苗生长势、叶面积、生物量等分析了球孢白僵菌定殖对牛蒡苗生长指标的影响，同时通过测定牛蒡苗中PPO、 PAL、 SOD、 CAT、POD的活性考察球孢白僵菌定殖对牛蒡苗抗性的影响。[结果]球孢白僵菌定殖能够显著促进牛蒡幼苗的生长能力并提高根、茎、叶组织中常见防御酶的活性。浸种48和72 h组牛蒡的生长势、叶面积、苗鲜重和生物量均高于对照组，浸种72 h组又优于浸种48 h组，在出苗第28 天时，牛蒡苗株高、叶面积、苗鲜重和生物量分别比浸种48 h组提高了2.19%、6.40%、12.08%和9.02%;球孢白僵菌定殖同样提高了牛蒡苗中相关的抗性防御酶活性，浸种48和72 h组牛蒡苗中各种酶活性均显著高于对照组，浸种72 h组牛蒡苗根中酶活性均显著高于浸种48 h组，但茎中PPO、POD、PAL、SOD活性显著高于浸种48 h组，但CAT活性显著低于浸种48 h，叶中PAL、SOD、CAT活性显著高于浸种48 h组，但PPO、POD活性差异不显著。[结论]球孢白僵菌定殖牛蒡苗可促进牛蒡的生长并增强牛蒡的抗性。

关键词 球孢白僵菌;牛蒡;苗期;定殖;生长特性

中图分类号 S476文献标识码 A文章编号 0517-6611（2021）10-0134-04

doi：10.3969/j.issn.0517-6611.2021.10.036

开放科学（资源服务）标识码（OSID）：

Effect of Colonization of Beauveria bassiana on Growth Characteristic of Burduck Seedling

MENG Yi1， ZHA Jin-hong1， ZHUWen-lan1，2et al

（1.College of Food （Biological） Engineering， Xuzhou University of Technology，Xuzhou，Jiangsu221111;2. Jiangsu Key Laboratory of Food Resources Development and Quality Safety， Xuzhou University of Technology， Xuzhou，Jiangsu221111）

Abstract [Objective] To study the effect of colonization of Beauveria bassiana on the growth characteristic in the burdock seedling. [Method] Beauveria bassiana was colonized in burdock seedling by soaking method. The growth potential， leaf area， biomass and the activity of PPO， PAL， SOD， CAT POD in the burdock seedling were detected to study the effect of colonization on the growth characteristic.[Result] Bassiana bassiana colonization could significantly promote the growth ability of burdock seedlings and enhance the activity of common defense enzymes in root， stem and leaf tissues. The growth potential， leaf area， seedling fresh weight and biomass of burdock in the soaking group for 48 and 72 h were higher than those in the control groups， and the soaking group for 72 h was superior to the soaking group for 48 h. The colonization of Bassiana bassiana also raised the defense enzyme activity of burdock seedlings. Enzyme activity of soaking 48 and 72 h groups were significantly higher than that of control groups， enzymes activity of burdock root in soaking 72 h group were significantly higher than that of soaking 48 h group， but PPO， POD， PAL， SOD activity of stem were significantly higher than that of soaking 48 h group， but CAT activity was significantly lower than that of soaking 48 h， and PAL， SOD， CAT activity of the leaves were significantly higher than that of 48 h group， but the PPO， POD activity were not significantly different. [Conclusion]The results indicated that colonization of Bassiana bassiana in burdock seedlings could promote the growth of burdock and enhance the resistance of burdock.

Key words Beauveria bassiana;Burdock;Seedling stage;Colonization;Growth characteristic

球孢白僵菌（Beauveria bassiana）是目前研究和应用最多的昆虫病原菌之一[1]，因其寄主昆虫分布广，因此被广泛应用于害虫防治[2-4]，在植物害虫生防应用中发挥着重要作用[5-6]，其可穿透病原昆虫体壁，并在昆虫体内不断繁殖增长，破坏虫体组织使其早期死亡。……