关慧龙

【摘要】 目的：探究乙肝孕婦病毒载量与Th1、Th2类细胞因子表达的关系。方法：选取2019年1月-2020年1月的100例乙肝孕妇为观察组，同时期的100例健康孕妇为对照组。检测并比较两组的血清Th1类细胞因子（IL-2、TNF-α及IFN-γ）及Th2类细胞因子（IL-4、IL-6及IL-10）表达情况，并比较观察组中不同病毒载量者的血清Th1、Th2类细胞因子表达情况，采用Spearman秩相关分析血清Th1、Th2类细胞因子与乙肝孕妇病毒载量的关系。结果：观察组的血清IL-2表达显著低于对照组，TNF-α、IFN-γ、IL-4、IL-6及IL-10表达均显著高于对照组，差异均有统计学意义（P<0.05）。观察组中病毒载量较高者的血清IL-2表达显著低于病毒载量较低者，TNF-α、IFN-γ、IL-4、IL-6及IL-10表达均显著高于病毒载量较低者，差异均有统计学意义（P<0.05）。观察组中不同病毒载量者的血清Th1、Th2类细胞因子表达比较，差异均有统计学意义（P<0.05）。Spearman秩相关分析显示，血清IL-2与乙肝孕妇病毒载量呈负相关，TNF-α、IFN-γ、IL-4、IL-6及IL-10与乙肝孕妇病毒载量呈正相关（P<0.05）。结论：乙肝孕妇病毒载量与Th1、Th2类细胞因子表达有密切的关系，应重视对乙肝孕妇上述方面的监测与调控。

【关键词】 乙肝 孕妇 病毒载量 Th1类细胞因子 Th2类细胞因子

Investigation on Relationship between Viral Loads and Expressions of Th1 and Th2 Cytokines of Pregnant Women with Hepatitis B/GUAN Huilong. //Medical Innovation of China， 2021， 18（12）： -181

[Abstract] Objective： To investigate the relationship between viral loads and expressions of Th1 and Th2 cytokines of pregnant women with hepatitis B. Method： A total of 100 hepatitis B pregnant women from January 2019 to January 2020 were selected as the observation group， and 100 healthy pregnant women during the same period were selected as the control group. The expressions of Th1 cytokines （IL-2， TNF-α and IFN-γ） and Th2 cytokines （IL-4， IL-6 and IL-10） in serum of the two groups were detected and compared. The expressions of Th1 and Th2 cytokines in serum of patients with different viral loads in the observation group were compared. Spearman rank correlation was used to analyze the relationship between serum Th1 and Th2 cytokines and viral load in pregnant women with hepatitis B. Result： The expression of serum IL-2 in the observation group was significantly lower than that in control group， and the expressions of TNF-α， IFN-γ， IL-4， IL-6 and IL-10 in the observation group were significantly higher than those in the control group， the differences were statistically significant （P<0.05）. In the observation group， the expression of serum IL-2 in patients with high viral load was significantly lower than that in patients with low viral load， and the expressions of TNF-α， IFN-γ， IL-4， IL-6 and IL-10 in patients with high viral load were significantly higher than those in patients with low viral load， the differences were statistically significant （P<0.05）. The expression of Th1 and Th2 cytokines in serum of patients with different viral loads in the observation group were compared， the differences were statistically significant （P<0.05）. Spearman rank correlation analysis showed that serum IL-2 was negatively correlated with viral loads in pregnant women with hepatitis B， TNF-α， IFN-γ， IL-4， IL-6 and IL-10 were positively correlated with viral load in pregnant women with hepatitis B （P<0.05）. Conclusion： The viral load of pregnant women with hepatitis B is closely related to the expression of Th1 and Th2 cytokines， so it is necessary to pay attention to the monitoring and regulation of the above aspects.

[Key words] Hepatitis B Pregnant women Viral loads Th1 cytokines Th2 cytokines

First-authors address： Fengcheng Hospital of Traditional Chinese， Fengcheng 118100， China

doi：10.3969/j.issn.1674-4985.2021.12.043

乙肝孕婦在临床多见，也是临床的重点研究方面。母婴传播作为我国乙肝传播的常见方式之一，临床对其研究繁多，而乙肝孕妇的病毒载量作为与疾病传染程度密切相关的方面，是研究的重中之重[1-2]。另外，Th1及Th2类细胞因子作为机体感染、炎性及免疫应激的重要反应指标，其在乙肝患者中的表达变化研究可见，但是在乙肝孕妇中的表达研究不足，而针对其与病毒载量的关系探究差异突出，因此本方面的探究需求及意义均较高[3-5]。本研究就乙肝孕妇病毒载量与Th1、Th2类细胞因子表达的关系进行探究，现报道如下。

1 资料与方法

1.1 一般资料 选取2019年1月-2020年1月的100例乙肝孕妇为观察组，同时期的100例健康孕妇为对照组。纳入标准：20～40岁;孕周>28周;单胎。排除标准：合并妊娠期高血压、妊娠期糖尿病等妊娠期疾病;合并创伤;合并其他感染;检查前进行相关抗病毒治疗;合并其他类型的肝炎;合并胎膜早破、胎盘早剥等情况;合并胎儿异常。患……