张柳莲 黄清铧 王丽宁 王庆福 梁磊 林明威

摘  要：为了筛选出最适合灵芝菌种复壮的培养基和灵芝菌种的保藏方法，通过添加不同含量的甘蔗渣提取液作为灵芝菌株复壮的培养基，观察灵芝菌丝在不同培养基下的生长速度和生长状况，并通过对灵芝菌种酶活力进行测定分析，比较几种不同保藏方法对灵芝菌种活力的影响。结果表明：马铃薯葡萄糖培养基（PDA）培养基添加甘蔗渣提取液能更好促进灵芝菌丝生长，添加100 g/L甘蔗渣提取液，赤灵芝GL102、紫灵芝GS63和GS15菌丝生长速率分别提高48%、70%和28%。与PDA保藏和石蜡油保藏培养基相比，甘蔗渣保藏的灵芝菌丝生长快、长势好，脱氢酶活力高。因此，甘蔗渣可用于灵芝菌种的复壮和保藏。

关键词：甘蔗渣;灵芝菌种;菌丝生长;培养基;脱氢酶活性

中图分类号：Q939.99      文献标识码：A

Effect of Bagasse on Growth of Ganoderma Mycelium and Evaluation of Strain Preservation

ZHANG Liulian1， HUANG Qinghua1， WANG Lining1， WANG Qingfu1*， LIANG Lei2， LIN Mingwei2

1. Institute of Bioengineering， Guangdong Academy of Sciences / Guangdong Provincial Engineering Laboratory of Biomass High Value Utilization， Guangzhou， Guangdong 510316， China; 2. Heyuan Provincial Academy of Sciences， Heyuan， Guangdong 517000， China

Abstract： In order to select the most suitable culture medium for the rejuvenation and preservation method of Ganoderma strains， by adding different contents of bagasse extract as the medium for the rejuvenation and observed hypha growth rate under different culture medium and growth conditions， and through the comparison of several different preservation methods on the vigor and the analysis of the enzyme activity. The results showed that the mycelium growth was better promoted by adding extraction solution of bagasse into PDA medium， and the mycelium growth rate of Ganoderma lucidum GL102， Ganoderma sinensis GS63 and GS15 increased by 48%， 70% and 28% respectively with the addition of 100 g/L bagasse extract. Compared with PDA preservation and paraffin oil preservation medium， bagasse preservation of Ganoderma mycelia grew fast， grew well and had high dehydrogenase activity.  It was concluded that bagasse can be used for the rejuvenation and preservation of Ganoderma strains.

Keywords： bagasse; ganoderma strains; mycelium growth; medium; dehydrogenase activity

DOI： 10.3969/j.issn.1000-2561.2021.04.019

靈芝属多孔菌科，灵芝属，是我国最著名的药食两用真菌，具有抗肿瘤[1]、保肝护肝[2]、增强人体免疫力[3]、调节血糖血压等多方面功效[4-5]。近年来，随着灵芝栽培行业的迅速发展，作为生产源头的菌种成为直接关系到产品产量和质量的关键因素。菌种保藏方法与效果作为种质资源研究的重要方面，是菌种质量的前提和保证[6-8]。建立经济有效的菌种保藏方法，不仅可以有效保存菌种的优良性状、保持其活力与产量，而且对于种质资源的保存与后续研究都有极其重要的作用。

甘蔗渣是制糖工业的重要副产品，约占榨蔗量的24%～27%。甘蔗渣含有丰富的纤维素、半纤维素、木质素和一定量的氮素[9]，可为多数食用菌提供生长代谢所需营养物质，目前有关甘蔗渣在食用菌栽培方面的相关研究集中在栽培方面[10-11]，鲜见甘蔗渣在食用菌菌种保藏方面的相关报道。……