姜莲 程韬 王影超 舒柯

【摘 要】 目的：为提升黔产迎春花药材的质量标准提供参考。方法：本研究采用显微鉴别及薄层色谱鉴别法对迎春花进行定性鉴别，同时根据《中国药典》2015年版规定的方法对其水分、总灰分、酸不溶性灰分及浸出物进行定量测定，以总黄酮为指标，通过系统的方法学考察，建立迎春花药材的UV含量测定方法。结果：迎春花药材显微特征、薄层色谱斑点均清晰明显。3批迎春花药材样品的水分测定结果为12.1%～13.5%，总灰分测定结果为5.0%～5.8%，酸不溶性灰分测定结果为0.7%～1.2%，浸出物测定结果为26.1%～30.2%，3批迎春花药材中总黄酮的含量为2.50%～3.56%。结论：本研究为提高迎春花药材质量标准提供参考。

【关键词】 迎春花;定性鉴别;总黄酮;含量测定

【中图分类号】R282   【文献标志码】 A    【文章编号】1007-8517（2021）05-0026-04

Quality Standard for Jasminum Nudiflorum from Guizhou Province

JIANG Lian CHENG Tao WANG Yingchao* SHU Ke

Guizhou Institute for Food and Drug Control，Guiyang 550004，China

Abstract：Objective To provide reference for improving the quality standard of Jasminum nudiflorum from Guizhou.Methods Jasminum nudiflorum was qualitatively identified by microscopic characteristics and TLC methods，and referring to Chinese Pharmacopoeia of 2015 edition，water content，total ash，acid-insoluble ash and extract were also determined.Using Total flavonoids as indicators，the content determination with UV in Total flavonoids was established through systematic methodological investigations respectively.Results The microscopic characteristics and the spots of TLC were clear and evident.The moisture，total ash，acid-insoluble ash and extract of 3 Jasminum nudiflorum samples varied in the ranges of 12.1%～13.5%，5.0%～5.8%，0.7%～1.2%，and 26.1%～30.2%，the contents of Total flavonoids in 3 batches of Jasminum nudiflorum were 2.50%～3.56%.Conclusion This study provides a reference for improving the quality standard of Jasminum nudiflorum.

Keywords：Jasminum Nudiflorum;Qualitative Identification;Total Flavonoids;Content Determination

迎春花，又称金腰带、黄素馨，为木犀科植物迎春花Jasminum nudiflorum Lindl.的干燥叶和花。春、夏、秋三季均可采摘，晾干或晒干。药用价值在我国历史悠久，主要功效为解毒消肿、清热利尿、止血止痛，用于发热头痛、小便热痛、跌扑损伤、外伤出血、口疮、痈疖肿痛、下肢溃疡[1]。产于贵州思南、贵阳、安顺等地。我国山西、陕西、甘肃、山东、河南、江苏、四川、云南也有分布[2]。王佩珩等[3-4]对迎春花的化合物进行分析，得到裂环烯醚萜类化合物、黄酮类化合物、迎春花黄色素、多糖类化合物、挥发油类和脂肪酸类等。为提高迎春花的质量控制水平，本实验建立显微鉴别、薄层鉴别、水分、总灰分、酸不溶性灰分、浸出物及总黄酮的含量测定，为迎春花的研究及质量控制提供依据。……