陈苑平 陈柯 许金森 郑淑霞

【摘 要】 目的：观察电针足三里对腹泻型肠易激综合征（IBS-D）大鼠内脏敏感性及腹瀉情况的影响。方法：将30只SD大鼠随机分为空白组、模型组及电针组，每组10只。模型组和电针组均采用慢性束缚应激联合番泻叶煎剂灌胃的方法建立IBS-D模型，造模2w。造模成功后，电针组采用电针足三里穴干预。观察各组大鼠的的精神状态、活动情况、毛发光泽度以及体重增长等情况，测算腹泻指数，并评估所有大鼠的内脏敏感性情况。结果：与空白组相比较，模型组及电针组干预前体重增长幅度较小（P<0.01），干预期之后电针组大鼠体重增长情况得到一定改善（P<0.01）。与空白组相比，模型组大鼠的结直肠扩张的容量阈值显著降低（P<0.01），与模型组相比，电针组大鼠的结直肠扩张容量阈值在电针干预后有所提高（P<0.05），且电针组大鼠的腹泻情况也有所好转（P<0.01）。结论：电针足三里穴能够改善IBS-D大鼠的易激惹、倦怠、体重增长缓慢的情况，改善大鼠的腹泻情况，降低内脏高敏感性。

【关键词】 电针;足三里;腹泻型肠易激综合征;内脏敏感性;腹泻指数

【中图分类号】R246   【文献标志码】 A    【文章编号】1007-8517（2021）05-0013-04

Effects of Electroacupuncture Zusanli on Visceral Sensitivity and Diarrhea in Rats with Diarrhea-type Irritable Bowel Syndrome

CHEN Yuanping1 CHEN Ke1 XU Jinsen2 ZHENG Shuxia2*

1. Fujian University of Traditional Chinese Medicine， Fuzhou 350108， China;

2. Fujian Academy of Chinese Medical Sciences， Fuzhou 350003， China

Abstract：Objective To observe the effect of electroacupuncture zusanli on visceral sensitivity and diarrhea in diarrhea-type irritable bowel syndrome （IBS-D） rats. Methods 30 SD rats were randomly divided into blank group， model group and electroacupuncture group， with 10 rats in each group. Both the model group and the electroacupuncture group adopted the method of chronic binding stress combined with senna lesf decoction is given orally to establish the IBS-D model， and the model was constructed for 2 weeks. After successful modeling， electroacupuncture group adopted electroacupuncture zusanli point intervention. The mental state， activity， hair gloss and weight gain of each group were observed， the diarrhea index was measured， and the visceral sensitivity of all rats was evaluated. Results Compared with the blank group， the body weight increase of the model group and the electroacupuncture group was smaller before intervention （P<0.01）， and the body weight increase of the electroacupuncture group was improved to some extent after intervention （P<0.01）. Compared with the blank group， the volume threshold of colorectal dilatation in the model group was significantly reduced （P<0.01）. Compared with the model group， the volume threshold of colorectal dilatation in the electroacupuncture group was improved after the electroacupuncture intervention （P<0.05）， and the diarrhea of the electroacupuncture group was also improved （P<0.01）. Conclusion Electroacupuncture at Zusanli point can improve the irritability， tiredness and slow weight gain in IBS-D rats， improve the diarrhea in rats， and reduce the visceral hypersensitivity.

Keywords：Electroacupuncture; Zusanli; Diarrhea-type Irritable Bowel Syndrome;Visceral Sensitivity;Diarrhea Index

腸易激综合征（Irritable Bowel Syndrome，IBS）是常见的消化系疾病，表现为腹痛或腹部不适，且伴随排便情况改变的功能性肠病，多为慢性、间歇性发作。IBS的发病率逐年增高，在IBS的亚型中又以腹泻型（IBS-D）最为常见，约占IBS发病率的3/4，因IBS-D容易反复发作，给患者生活及心理造成一定的影响[1]。研究认为，IBS的发病机制与胃肠道动力异常以及内脏高敏感性存在联系[2]。研究调查表明，目前西药对该疾病的治疗存在一定的局限性，而针灸对该病的治疗则有一定的优势[3]。本实验通过电针大鼠足三里，观察电针足三里对IBS-D模型大鼠的内脏敏感性以及大鼠腹泻情况的影响，初步探讨电针足三里对于IBS-D的疗效及其效应机制。

1 实验材料

1.1 实验动物 健康的雄性SD大鼠30只，体重（200～230）g，动物许可证号： SCXK（沪）2018-0006（上海西普尔-必凯实验动物有限公司），实验前先适应性饲养于福建中医药科学院动物房（普通级）7 d，控制室内温度为 23～26 ℃，相对湿度为50%～70%。……