郭延林 张树泉 王小亮

【摘 要】 目的：评价益肾通脉方改善脑缺血再灌注大鼠神经功能方面的作用。方法：将雄性SD大鼠随机分为假手术组、模型组、益肾通脉低剂量组和益肾通脉高剂量组;采用线栓法制备大脑中动脉梗死缺血再灌注模型（缺血时间为1 h），再灌注24 h后对造模成功大鼠分别给药，假手术组、模型组给予等量蒸馏水。观察各组大鼠神经功能缺损变化及血清MDA、SOD、HIF-1α、ET-1、eNOS、NO水平。結果：益肾通脉低剂量组大鼠的神经功能缺损评分在第2、3、4、5天显著高于假手术组（P<0.05），但与模型组无统计学差异（P>0.05）。益肾通脉高剂量组大鼠的神经功能缺损评分在前两天时明显高于假手术组，但低于模型组。与假手术组相比，SOD的含量在模型组和益肾通脉低剂量组中显著下降，而HIF-1α却显著增加。结论：益肾通脉方可改善脑缺血再灌注大鼠神经功能，升高血清SOD水平，降低血清HIF-1α和eNOS含量，提示其脑保护机制可能与抗氧化、减轻炎症反应有关。

【关键词】 益肾通脉方;脑缺血再灌注;神经功能

【中图分类号】R285.5 【文献标志码】 A【文章编号】1007-8517（2021）01-0013-04

Preliminary Study of Yishen Tongmai Recipe on Improving Neurological Function of Rats with Cerebral Ischemia-reperfusion

GUO Yanlin ZHANG Shuquan* WANG Xiaoliang

Department of encephalopathy， TCM hospital of Taian， Taian 271000，China

Abstract：Objective To evaluate the effect of Yishentongmai Recipe on improving neurological function in rats with cerebral ischemia-reperfusion.Methods male SD rats were randomly divided into sham operation group， model group， Yishentongmai low-dose group and Yishentongmai high-dose group;The middle cerebral artery infarction （MCAO） ischemia-reperfusion model was prepared by thread embolism method （ischemia time was 1h）. After 24h reperfusion， the rats were given medicine respectively， and the sham operation group and model group were given equal amount of distilled water.The changes of nerve function defect and serum levels of MDA， SOD， HIF-1 α， ET-1， eNOS and NO were observed.Results the scores of neurological deficit in the Yishentongmai low-dose group were significantly higher than those in the sham operation group on the 2nd， 3rd， 4th and 5th days （P<0.05）， but there was no significant difference between the two groups （P>0.05）.In the first two days， the neurological deficit score of the high-dose Yishentongmai group was significantly higher than that of the sham operation group， but lower than that of the model group.Compared with the sham operation group， the content of SOD decreased significantly in the model group and Yishentongmai low-dose group， but HIF-1 α increased significantly.Conclusion Yishentongmai decoction can improve the neurological function of rats with cerebral ischemia-reperfusion， increase the level of SOD in serum， and decrease the content of HIF-1 α and eNOS in serum. It suggests that the brain protection mechanism of Yishentongmai decoction may be related to antioxidation and reducing inflammatory reaction.

Keywords：Yishen Tongmai Recipe; Cerebral Ischemia-Reperfusion; Nerve Function

脑梗死为临床常见多发疾病，是我国发病率、致残率、病死率最高的疾病之一，给家庭和社会带来了极大的负担。超早期静脉溶栓和机械取栓治疗是目前急性脑梗死具有循证医学证据的治疗方法，脑血管再通治疗可挽救“缺血半暗带”，使其向正常组织转化或稳定不继续恶化[1]。超早期血管开通治疗后可通过“氧化应激”“炎性反应”等多种渠道发生“缺血-再灌注（I/R）”损伤[2]，加重脑组织损伤，导致神经功能缺损加重。……