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低温下7个草莓品种的抗性生理指标比较

2021-05-12杨馥霞汤玲贺欢孔芬王卫成

甘肃农业科技 2021年4期

杨馥霞 汤玲 贺欢 孔芬 王卫成

摘要:以7个草莓新品种为试材,在-15~4 ℃范围内设置7个温度梯度处理,测定草莓叶片叶绿素、可溶性糖、游离脯氨酸及丙二醛(MDA)含量,以评价其抗寒性。结果表明,低温处理后,宁玉叶片中葉绿素含量最高;隋珠、宁玉的可溶性糖含量较高,但各品种间差异不明显。脯氨酸含量受到影响因素较多,不能从含量上判断与抗寒力强弱的相关性;京藏香的MDA含量一直较高,宁玉的MDA含量较低且稳定。宁玉的抗寒力最强,京藏香较弱,其他品种介于两者之间。

关键词:草莓;抗寒性;可溶性糖;MDA;游离脯氨酸

中图分类号:S668.4       文献标志码:A       文章编号:1001-1463(2021)04-0014-04

doi:10.3969/j.issn.1001-1463.2021.04.005

Comparison of Resistant Physiological Index among Seven New Strawberry Cultivars after Low Temperature Treatment

YANG Fuxia, TANG Ling, HE Huan, KONG Fen, WANG Weicheng

(Institute of Fruit and Floriculture, Gansu Academy of Agricultural Sciences, Lanzhou Gansu 730070, China)

Abstract:In order to evaluate cold tolerance of seven strawberry cultivars, we measured the contents of chlorophyll, soluble sugar, proline and malondialdehyde (MDA) after the plants were treated at seven different temperatures from -15 ℃ to 4 ℃. The results showed that after low temperature treatment, the chlorophyll content was highest in Ningyu leaves, the soluble sugar content of Suizhu and Ningyu was higher, but the difference was not obvious among the cultivar. Proline content is affected by many factors, so it cannot be judged from the content of the correlation with cold resistance strength; the MDA content of Jingzangxiang was always high, Ningyu was low and stable. The cold tolerance of Ningyu is strongest and of Jingzangxiang is weakest, and of other cultivars is intermediate.

Key words:Strawberry; Cold resistance; Soluble sugar; MDA; Proline

甘肃位于中国西北地区,冬季寒冷干燥。随着草莓生产规模的扩大,低温伤害成为影响草莓栽培的因素之一,选择抗寒性强、适应性强的品种成为当地育种及引种的目标[1 ]。近年来,甘肃省陆续引进一系列草莓新品种[2 ],但这些品种在寒旱条件下适应性如何,需要采用与抗寒相关的生理指标进行评价。我们前期对部分品种低温半致死温度进行了测定[3 ],现通过对草莓叶片中叶绿素含量、可溶性糖含量、游离脯氨酸含量及MDA含量变化的测定,进一步评价草莓品种红颜、甘露、隋珠、京藏香、宁玉、蒙特瑞、圣安德瑞斯的抗寒性,以期为筛选适应甘肃省寒旱生态环境栽培的草莓品种及抗寒亲本的选择提供参考。

1   材料与方法

1.1   供试材料

供试草莓品种为红颜(Benihoppe)、甘露(Ganlu)、隋珠(Suizhu)、京藏香(Jingzangxiang)、宁玉(Ningyu)、蒙特瑞(Monterey)、圣安德瑞斯(San Andreas),均由甘肃省农业科学院林果花卉研究所提供。……

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