张栩浩 张贝 郑薇薇 张宏宇

摘要 ：柑橘全爪螨Panonychus citri是湖北省柑橘园中常见害螨，可造成柑橘树落花、落叶、落果。在生产无公害水果时利用生物防治技术来控制果园中的害虫是一项重要技术。江原鈍绥螨Amblyseius eharai在湖北省柑橘园中是柑橘全爪螨的优势捕食螨。利用江原钝绥螨控制柑橘全爪螨的技术中需要考虑到江原钝绥螨的低温贮藏问题。本试验研究了低温贮藏过程中的温度和冷藏时间对江原钝绥螨的影响。结果表明，江原钝绥螨在5、7、9℃这3种冷藏温度下存活率和7 d内产卵数量不同。冷藏5～15 d内，不同温度冷藏后存活率无显著性差异，但用7℃冷藏可使该螨有较高的产卵量，产卵量在17.1～18.1粒之间;冷藏20～60 d内用5℃冷藏可使该螨有较高的存活率和较高的产卵量，其存活率在26.7%～66.7%之间，产卵量在13.3～17.1粒之间;由此可见，冷藏时间在15 d内用7℃冷藏效果最佳;冷藏时间为20～60 d，用5℃冷藏会使该螨的存活率和冷藏后产卵量都最佳。本研究可为江原钝绥螨低温贮藏技术奠定理论基础，对害螨生物防治具有重要的实践指导价值。

关键词 ：江原钝绥螨; 低温贮藏; 存活率; 产卵量

中图分类号： S 476.2

文献标识码： A

DOI： 10.16688/j.zwbh.2019535

Cold storage technology of Amblyseius eharai

ZHANG Xuhao#， ZHANG Bei#， ZHENG Weiwei*， ZHANG Hongyu*

（College of Plant Science & Technology， Institute of Urban and Horticultural Entomology， Huazhong Agricultural University， Wuhan 430070， China）

Abstract ：Panonychus citri is a common pest mite in citrus orchards in Hubei province， causing blossom loss， fallen leaves and fallen fruits in citrus. The use of biocontrol techniques to manage pests in orchards plays an important role in the production of pollutionfree fruits. Amblyseius eharai is a dominant predator mite of P.citri in citrus orchards in Hubei. The cold storage of A.eharai needs to be considered in applying A.eharai to control of P.citri. This study was aimed to investigate the effects of temperature and cold storage time on A.eharai in the cold storage process. The results showed that A.eharai exhibited different survival rates and amounts of oviposition in seven days under three cold temperatures （5， 7℃ and 9℃）. There was no significant difference in the survival rate of the mite after 5-15 d of cold storage at different temperatures， but the mites had a higher egglaying capacity when stored at 7℃. The number of eggs was approximately 17.1-18.1. Refrigeration at 5℃ for 20-60 d gave rise to higher survival rates （26.7% to 66.7%） and more egg deposition （13.3 to 17.1）. Therefore， 7℃ is the best choice within 15 d of cold storage， but 5℃ can result in the best survival rate and the most egg deposition if stored for 20-60 d. This study lays a theoretical foundation for the cold storage technology of A.eharai， and provides an important practical guidance for biological control of pest mites.

Key words ：Amblyseius eharai; refrigeration; survival rate; amount of oviposition

柑橘全爪螨Panonychus citri是一种广泛分布于我国南方各地的常见害螨。近年来随着全球气候变暖，柑橘全爪螨的为害期变长，发育速度加快，一年可发生15～18代。柑橘全爪螨的幼螨、若螨、成螨都能吸食柑橘叶片、嫩枝、幼果等部位的汁液。使受害部位由绿转白，更可导致落叶、落花、落果[1]。柑橘全爪螨的传统防治方法大多使用化学药剂。……