刘军 陈田 汤记路 刘媛媛 杨占秋

摘要 目的：观察小儿宝泰康颗粒对流感病毒感染细胞的抑制作用。方法：通过细胞培养技术观察不同浓度的小儿宝泰康颗粒对Madin-Darby犬腎（MDCK）细胞的细胞毒性作用，采用MTT法并结合CPE法检测细胞成活率和病毒抑制率，计算药物的抗病毒指数TI，以利巴韦林作为阳性对照药物，评价该药物对甲、乙型流感病毒的抗病毒活性。结果：小儿宝泰康颗粒对MDCK细胞的半数细胞毒性浓度（CC50）为5.6 mg/mL，对甲型H1N1流感病毒感染MDCK细胞的半数有效浓度（EC50）为2.1 mg/mL，而对乙型流感病毒的EC50为1.44 mg/mL，抗病毒指数分别为2.64和3.89。在阳性对照药物实验中，利巴韦林在200 μg/mL时，对甲、乙型流感病毒的抑制率均达到70%以上。结论：小儿宝泰康颗粒是一种对不同型流感病毒感染有抗病毒作用的药物，有临床应用前景和进一步研究的价值。

关键词 小儿宝泰康颗粒;流感病毒;MTT法;CPE法;抗病毒指数;细胞实验;病毒抑制率;细胞毒性;半数有效浓度

Study on Antiviral Activity of Xiao′er Baotaikang Granuels Against Influenza Virus in Vitro

LIU Jun1，CHEN Tian2，TANG Jilu1，LIU Yuanyuan 1，YANG Zhanqiu1

（1 Institute of Medical Virology，School of Basic Medicine，Wuhan University，Wuhan 430071，China; 2 Department of Clinical Lab，Hubei Provincial Hospital of Integrated Traditional Chinese and Western Medicine，Wuhan 430015，China）

Abstract Objective：To investigate the antiviral activity of Xiao′er Baotaikang Granuels on influenza virus in vitro.Methods：We observed the cytotoxic effect of different concentrations of Xiaoer Baotaikang Granules on Madin-Darby canine kidney（MDCK） cells by cell culture technology，used MTT method combined with CPE method to detect cell survival rate and virus inhibition rate，calculated the drug′s antiviral index TI，and used ribavirin as positive control drug to evaluate the antiviral activity of the drug against influenza A and B viruses.Results：The 50% cytotoxic concentration （CC50） of Xiaoer Baotaikang Granules on MDCK cells was 5.6 mg/mL，and the 50% effective concentration （EC50） for MDCK cells infected by influenza A H1N1 virus was 2.1 mg/mL，while for influenza B viruses The EC50 was 1.44 mg/mL，and the antiviral indexes were 2.64 and 3.89，respectively.In the positive control drug experiment，when ribavirin was 200 μg/mL，the inhibition rate of influenza A and B viruses reached more than 70%.Conclusion：Xiaoer Baotaikang Granules are a kind of antiviral drugs that have antiviral effects on different types of influenza virus infections.It has clinical application prospects and further research value.

Keywords Xiaoer Baotaikang Granules; Influenza virus; MTT method; Cytopathologic effect assay; Antiviral index; Cell experiment; Inhibition rate;Cytotoxicity; 50% effective concentration

中图分类号：R254.3文献标识码：Adoi：10.3969/j.issn.1673-7202.2021.23.018

在以发热为主要症状的呼吸道感染疾病中，流感病毒仍是最为常见的致病病原体[1]。由于流感病毒易发生变异，常出现新亚型病毒株传播流行。因此，人类尚不能完全控制流感病毒引起的病毒性传染病，历史上曾数次出现世界性大流行。如2009年底在美国和全球范围内爆发的新型甲型H1N1流感，当时无有效疫苗进行预防和控制其传播而造成大流行;而目前全球每年有近500万人感染流感病毒，其中至少有25～50万人死亡[2-3]。流感病毒（Influenza Virus）属于正粘病毒科（Orthomyxoviridae）成员，根据病毒的宿主类型，分为人流感病毒、禽流感病毒和动物流感病毒。……