梁彪 高家治 徐朝辉 王建东 施展

摘要 目的：通过网络药理学探讨淫羊藿增强免疫功能可能的作用机制。方法：通过中药系统药理学数据库与分析平台（TCMSP）检索淫羊藿的活性成分和靶点。利用Cytoscape 3.7.1软件构建“药物-活性成分-靶点”网络图。通过人类基因数据库（GeneCards）检索免疫抑制的相关靶点，利用STRING数据库进行蛋白质-蛋白质相互作用（PPI）网络分析，利用DAVID平台进行基因本体（GO）富集分析和京都基因和基因组百科全书（KEGG）富集通路分析。结果：筛选出淫羊藿21个有效活性成分，179个潜在靶点。GO生物过程共562条，包括信号转导、炎症反应、凋亡过程等;KEGG信号通路共120条，主要涉及癌症途径、MAPK信号通路、PI3K-AKT信号通路、TNF信号通路等。结论：淫羊藿的活性成分通过作用于AKT1、TP53、IL6、VEGFA等靶点，参与信号转导等生物過程，调控癌症途径、TNF信号通路等多个途径发挥增强免疫功能的作用。

关键词 淫羊藿;免疫功能;网络药理学;作用机制;成分;靶点;富集分析;信号通路

Study on the Mechanism of Herba Epimedii Enhancing Immune Function Based on Network Pharmacology

LIANG Biao，GAO Jiazhi，XU Zhaohui，WANG Jiandong，SHI Zhan

（Putuo Hospital，Shanghai University of Traditional Chinese Medicine，Shanghai 200062，China）

Abstract Objective：To explore the mechanism of Herba Epimedii to enhance immune function based on network pharmacology.Methods：The components and targets of Herba Epimedii were obtained by searching for Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform.Cytoscape 3.7.1 was used to build a network among drug，components and targets（drug-component-target network）.Targets for immunosuppression were screened through GeneCards.Protein interaction network analysis was performed by using the STRING database.Gene ontology analysis and Kyoto encyclopedia of genes and genomes signal pathway enrichment analysis were performed by using the DAVID platform.Results：A total of 21 active ingredients and 179 potential targets of Herba Epimedii were screened.There were 562 GO biological process，including signal transduction，inflammatory response，apoptotic process，etc.There were 120 KEGG signaling pathways，including Pathways in cancer，MAPK signaling pathway，PI3K-AKT signaling pathway，TNF signaling pathway.Conclusion：The active ingredients of Herba Epimedii play a role in enhancing immune function by acting on AKT1，TP53，IL6，VEGFA and other targets，participating in biological processes such as signal transduction，regulating cancer pathways，TNF signaling pathways and other pathways.

Keywords Herba Epimedii; Immune function; Network pharmacology; Mechanism; Components; Target; Enrichment analysis; Signaling pathway

中图分类号：R285文献标识码：Adoi：10.3969/j.issn.1673-7202.2021.23.011

淫羊藿又名仙灵脾，是临床常用补益类中药，根据《中华人民共和国药典》记载，淫羊藿具有补肾阳、强筋骨、祛风湿等功效，《神农本草经》记载：“味辛寒。主阴痿绝伤，茎中痛，利小便，益气力，强志……”并将其列为中品。研究表明，淫羊藿可以增强机体的免疫功能，对免疫器官、巨噬细胞、T淋巴细胞等均具有一定的影响[1]。目前关于中药及复方研究方法往往具有一定的局限性，难以更加全面地阐述其药效物质基础和作用机制。本研究运用网络药理学的研究方法，探讨淫羊藿增强免疫功能的潜在作用靶点及相关通路，以期为临床应用和进一步的实验研究提供一定的理论依据。