周建宏 张艳丽 张利斌

[摘要] 目的 探討TAZ（Transcriptional coactivator with PDZ-binding- motif）mRNA在非小细胞肺癌（NSCLC）组织中的表达及其临床意义。方法 选择2010年6月至2013年1月在云南省第一人民医院胸外科手术切除的62例经病理确诊的非小细胞肺癌组织为研究组，选择癌旁组织（距癌组织>5 cm）和21例肺良性肿瘤组织为对照组，采用实时荧光定量PCR法检测TAZmRNA表达量。 结果 ①实时荧光定量PCR法检测到TAZ mRNA的表达量在非小细胞肺癌组织高于癌旁/肺良性肿瘤组织，差异有统计学意义（P=0.027/0.016<0.05）。②非小细胞肺癌患者癌组织中TAZmRNA表达量在肿瘤大小、分化程度和p-TNM分期中差异有统计学意义（P=0.007/0.000/0.024<0.05）。结论 TAZ可能参与非小细胞肺癌的发生、发展;可作为NSCLC预后及转移的评价指标、提供非小细胞肺癌治疗的靶点。

[关键词] 非小细胞肺癌;mRNA;转录共激活因子;实时荧光定量PCR

[中图分类号] R734.2          [文献标识码] A          [文章编号] 1673-9701（2021）36-0005-04

The expression and clinical significance of TAZmRNA in non-small cell lung cancer tissues

ZHOU Jianhong1   ZHANG Yanli2   ZHANG Libin3

1.Department of Thoracic and Cardiovascular Surgery，the People's Hospital of Chuxiong Yi Autonomous Prefecture in Yunnan Province，Chuxiong   675000，China;2.The First Department of Anesthesia，the People's Hospital of Chuxiong Yi Autonomous Prefecture in Yunnan Province，Chuxiong   675000，China; 3.Department of Thoracic Surgery， the First People's Hospital of Yunnan Province， Kunming   650032， China

[Abstract] Objective To investigate the expression and clinical significance of TAZ （Transcriptional coactivator with PDZ-binding-motif） mRNA in non-small cell lung cancer （NSCLC） tissues. Methods A total of 62 pathologically confirmed non-small cell lung cancer tissues previously resected at the Department of Thoracic Surgery of the First People's Hospital of Yunnan Province from June 2010 to January 2013 were selected as the study group. The tissues adjacent to cancer （more than 5 cm from the cancer tissue） and 21 benign lung tumor tissues were selected as the control group. The expression of TAZ mRNA was detected by real-time quantitative PCR. Results ①The expression level of TAZ mRNA in the non-small cell lung cancer tissues was higher than that in paracancerous/benign lung tumors by real-time fluorescent quantitative PCR， and the difference was statistically significant （P=0.027/0.016<0.05）.②The expression of TAZ mRNA in non-small cell lung cancer tissues was significantly correlated with tumor size， differentiation， and p-TNM stage（P=0.007/0.000/0.024<0.05）. Conclusion TAZ may be involved in the occurrence and development of non-small cell lung cancer. It may be used as a prognostic and metastatic evaluation index of NSCLC and provide a therapeutic target for lung cancer.

[Key words] Lung cancer; mRNA; Transcriptional coactivator; Real-time quantitative PCR

目前肺癌是全世界发病率和死亡率最高的肿瘤[1-2]，其中约85%患者为非小细胞肺癌[3]，早期肺癌的治疗效果已经突显，而对于晚期及进展期肺癌的治疗仍然是难题及研究重点。转移是癌细胞从原发肿瘤扩散到不同器官，是癌症相关死亡的主要原因，对转移相关的靶向治疗有望成为提高肿瘤患者生存率的有效策略;而转移是一个复杂的过程，包括不同的步骤，癌细胞需要在每个步骤中获得不同的能力，并受到多种细胞信号通路的调控[4]。TAZ/WWTR1（Transcriptional coactivator with PDZ-binding motif/WW-domain containing transcription regulator 1 WWTR1）即具有PDZ结合域的转录共刺激因子，是一种转录辅激活物，参与体内细胞增殖、凋亡调控及多种器官的发育。研究发现，TAZ在多种肿瘤中表达升高，作为致癌因子参与肿瘤的发生、发展[5]。……