付菲菲 万守明

【摘 要】目的：比较酶联免疫吸附试验（ELISA）和电化学发光免疫法（ECLIA）在孕妇乙型肝炎表面抗原（HBsAg）检测中的应用价值。方法：选择我院近年来妇产科门诊收治的468例孕妇作为研究对象，分别采用ELISA法和ECLIA法检测孕妇的乙型肝炎表面抗原，比较两种检测方法的检测结果。结果：ELISA法和ECLIA法HBsAg阳性检出率分别为3.85%和3.42%。两种方法联合检测检出HBsAg阳性例数17例，阳性检出率为3.63%。ELISA法检测HBsAg的诊断敏感性和特异性分别为94.12%和99.56%，ECLIA法检测HBsAg的诊断敏感性和特异性分别为88.24%和99.78%（450/451），两种检测方法诊断敏感性和特异性之间的差异无统计学意义（P>0.05）。结论：酶联免疫吸附试验和电化学发光免疫法对于孕妇乙型肝炎表面抗原的检出率均较高，联合检测可进一步提高检出率。

【关键词】孕妇；乙型肝炎表面抗原（HBsAg）；酶联免疫吸附试验（ELISA）；电化学发光免疫法（ECLIA）；检验

Comparison of enzyme linked immunosorbent assay and electrochemiluminescence

immunoassay in the detection of hepatitis B surface antigen in pregnant women

Fu Feifei， Wan Shouming

Department of Laboratory， Dongtai maternal and Child Health Hospital， Dongtai Jiangsu 224299，China

【Abstract】Objective： To compare the application value of enzyme-linked immunosorbent assay （ELISA） and electrochemiluminescence immunoassay （ECLIA） in the detection of hepatitis B surface antigen （HBsAg） in pregnant women.Methods： 468 pregnant women admitted to the obstetrics and gynecology clinic of our hospital in recent years were selected as the research object. The HBsAg of pregnant women was detected by ELISA and ECLIA respectively， and the detection results of the two methods were compared.Results：the positive rates of HBsAg by ELISA and ECLIA were 3.85% and 3.42% respectively. The number of HBsAg positive cases detected by the two methods was 17， and the positive detection rate was 3.63%. The diagnostic sensitivity and specificity of ELISA for HBsAg were 94.12% and 99.56%， respectively. The diagnostic sensitivity and specificity of ECLIA for HBsAg were 88.24% and 99.78% （450/451）， respectively. There was no significant difference between two methods（P>0.05）.Conclusion： the detection rate of HBsAg in pregnant women is high by enzyme-linked immunosorbent assay and electrochemiluminescence immunoassay. The combined detection can further improve the detection rate.

【Key？Words】Pregnant women； Hepatitis B surface antigen （HBsAg）； Enzyme linked immunosorbent assay （ELISA）； Electrochemiluminescence immunoassay （ECLIA）； Test

乙型肝炎表面抗原（HBsAg） 是乙型肝炎病毒（HBV）的一種外壳蛋白，其本身并不具有传染性，但HBsAg的存在可提示HBV的存在，HBsAg阳性是HBV感染的重要标志[1]。母婴传播是婴幼儿感染HBV的主要方式，因此对于妊娠期妇女行HBsAg检测意义重大[2]。酶联免疫吸附试验（ELISA）和电化学发光免疫法（ECLIA）是目前检测HBsAg的常用方法，为进一步比较两种检测方法在孕妇HBsAg检测中的应用价值，本文将做如下研究。

1.1 一般资料

选择我院2018年5月至2021年7月间门诊妇产科收治的468例孕妇作为研究对象，所有孕妇均为在我院分娩的活产儿孕妇，孕妇签署知情同意书。排除凝血功能异常，合并感染性疾病以及临床资料不完整者。……