外源水杨酸和萘乙酸对红芸豆幼苗抗旱生理的影响
2021-01-05马仲炼赵玉仙刘健君田虹马永翠
马仲炼 赵玉仙 刘健君 田虹 马永翠



摘要 以红芸豆(地豆王)为材料,采用15%聚乙二醇(PEG)模拟干旱胁迫,研究外源水杨酸(salicylic acid,SA,100 mg/L)和萘乙酸(α-naphthaleneacetic acid,NAA,0.015 mmol/L)对红芸豆幼苗抗旱性的影响。结果表明,干旱条件下,喷施外源NAA、SA处理均能有效减少叶片水分流失,维持叶片相对含水量在较高水平,在干旱胁迫24 h后,叶片相对含水量仍高达81.30%、77.00%。SA、NAA处理可有效减缓MDA含量的积累,与CK处理相比分别低48.20%、49.12%;24 h内NAA较CK处理总叶绿素含量增加40.63%,在干旱胁迫36 h内SA可使红芸豆叶绿素a含量增加,但SA、NAA会造成可溶性蛋白质含量的降低,但总体而言,NAA、SA处理能提高红芸豆幼苗的抗旱能力。
关键词 红芸豆;水杨酸;萘乙酸;抗旱性
中图分类号 S643.1 文献标识码 A
文章编号 0517-6611(2021)24-0067-03
doi:10.3969/j.issn.0517-6611.2021.24.015
Effects of Exogenous Salicylic Acid and Naphthalic Acid on Drought Resistance of Red Kidney Bean Seedlings
MA Zhong-lian,ZHAO Yu-xian,LIU Jian-jun et al (College of Agronomy and Life Sciences,Zhaotong University,Zhaotong,Yunnan 657000)
Abstract The effects of salicylic acid (SA,100 mg/L) and α-Naphthaleneacetic acid (NAA,0.015 mmol/L) on physiological characters of red kidney bean (land bean king) under drought stress of 15% polyethylene glycol (PEG)during bean seedlings were studied. The results showed that spraying exogenous NAA,SA under the drought conditions could effectively reduce the loss of leaf water and maintain the relative water content of the leaves at a high level. After 24 h of the drought stress,the relative water content of the leaves was still high,which was 81.30% and 77.00%. SA and NAA processing could effective reduce the accumulation of MDA contents,which was 48.20% and 49.12% lower than that processed by CK respectively. Within 24 h of the drought stress,NAA could increase the total chlorophyll content,which was 40.63% higher than that processed by CK respectively. Within 36 h of the drought stress, SA could increase the content of chlorophyll a,but SA and NAA would reduce the soluble protein content.In general,NAA and SA processing could improve the drought resistance ability of red kidney bean seedlings.
Key words Red kidney bean;Salicylic acid;Naphthalene acetic acid;Drought resistance
基金項目 云南省一流课程植物生理学线下课程项目。
作者简介 马仲炼(1988—),男,云南昭阳人,讲师,从事作物逆境栽培研究。*通信作者,教授,从事作物病虫害生理研究。
收稿日期 2021-04-12
云南属热带、亚热带季风气候,全年降水不均,特别是春季干旱少雨,蒸发旺盛,日温变化大,这使云南的蔬菜种植面临严峻考验,其中红芸豆作为云南广泛种植的蔬菜同样受到干旱影响。近年来使用外源生长物质提高植物的抗旱性已成为热点,其中水杨酸(salicylic acid,SA),即邻羟基苯甲酸,是一种脂溶性有机酸,为白色结晶粉状物,是植物体内产生的简单酚类化合物,是一种新的植物内源激素,对缓解植物遭受逆境胁迫具有重要作用,目前在许多植物上运用并表现出良好的抗性。
SA参与植物的多种生理生化过程,能够诱导植物产生抗旱、抗盐碱等多种生理性状。……
