王宇卿 耿榕徽 张须学

摘要 目的：以艾叶中桉油精、樟脑、龙脑3种有效成分为指标，评价“宛艾”及其他产地艾叶的质量，为艾叶质量控制及道地药材基地建设提供技术支持。方法：采用安捷伦HP-5毛细管色谱柱（30 m×0.32 mm×0.25 μm）和氢火焰离子化检测器（FID检测器），气相色谱程序升温法测定。结果：32个产地艾叶中3种成分含量差异显著，南阳桐柏艾叶桉油精含量较高，南阳镇平艾叶樟脑含量较高，河北安国艾叶龙脑含量较高。结论：“宛艾”尤其是桐柏艾叶含量较高，本方法快捷简便，重复性好。

关键词 艾叶;桉油精;樟脑;龙脑;含量测定;气相色谱;质量控制;挥发性成分

Abstract Objective：To use 3 components of eucalyptus，camphor and borneol to evaluate Artemisia argyi from “wan ai” and other producing areas，providing technical support for the quality control of Artemisia argyi and the construction of genuine medicinal materials base.Methods：Agilent HP-5 capillary column（30 m×0.32 mm×0.25 μm）and FID detector（hydrogen flame ionization detector）were used.Determination was by Gas Chromatography Temperature Program.Results：There were significant differences in the content of the 3 components in the 32 producing areas.The content of eucalyptus in Nanyang Tongbai Artemisia argyi leaves was higher，the content of camphor in Nanyang Zhenping Artemisia argyi leaves was higher，and the content of borneol in Hebei Anguo Artemisia argyi leaves was higher.Conclusion：The method is simple and reproducible，it can be used for quality evaluation of Artemisia argyi leaves.Quality research of Artemisia argyi leaves provides technical support for the production base of genuine medicinal materials in Nanyang area.

Keywords Artemisia argyi leaves; Eucalyptol; Camphor; Borneol; Content determination; Gas chromatography; Quality control; Volatile components

中圖分类号：R282.6 文献标识码：A doi：10.3969/j.issn.1673-7202.2020.22.005

艾叶为菊科植物艾Artemisia argyi Levl.et Vant.的干燥叶，具有温经止血、散寒止痛，祛湿止痒之功效[1]。临床常用于妇科疾病、呼吸系统疾病、消化系统疾病以及外科疾病[2-5]。艾草在我国各地均有分布，南阳处于亚热带向暖温带的过渡地带，气候温暖湿润，适宜艾草生长，特别是南阳下辖的几个主产区：桐柏、唐河等地，产出的艾草质优量大，目前已成为全国最大的艾草输出基地，外界称南阳地区所产的艾草为“宛艾”。艾草主产地除南阳外还有蕲春、宁波、安国等地[2，5]。由于产地广泛、品种及气候差异造成了各地艾叶质量良莠不齐，因此对艾叶中主要有效成分的含量进行比较研究显得尤为重要。为了科学评价艾叶质量及开发道地艾叶生产基地，本实验采集了全国32个产地的艾叶样品，进行其主要有效成分的比较研究。……