王文通 江汉美 甘剑锋

摘要：采用顶空固相微萃取（HS-SPME）与气质联用法（GC-MS）对川芎茶调制剂不同剂型的挥发性成分进行比较。结果表明，在川芎茶调颗粒中共分离出24个峰，鉴定出16种成分，占其总挥发性成分的89.13%;在川芎茶调片中分离出63个峰，鉴定出46种成分，占其总挥发性成分的80.91%;在川芎茶调丸（浓缩丸）中分离出94个峰，鉴定出69种成分，占其总挥发性成分的90.10%;在川芎茶调丸（水丸）中分离出101个峰，鉴定出68种成分，占其总挥发性成分的78.50%。川芎茶调制剂的不同剂型的挥发性成分和相对百分含量存在一定的差异。

关键词：川芎茶调制剂;剂型;HS-SPME-GC-MS;挥发性成分

Abstract： HS-SPME-GC-MS was used for analysis the volatile components of Chuanxiong Chatiao preparations in different dosage forms. The results showed that 16 components were identified in the 24 peaks separated by Chuanxiong Chatiao granule， which accounted for 89.13% of the total volatile components. 46 components were identified in the 63 peaks separated by Chuanxiong Chatiao tablet， which accounted for 80.91% of the total volatile components. 69 components were identified in the 94 peaks separated by Chuanxiong Chatiao pill （concentrated pills）， which accounted for 90.10% of the total volatile components. 68 components were identified in the 101 peaks separated by Chuanxiong Chatiao pill （water-bindered pills）， which accounted for 78.50% of the total volatile components. The volatile components in different dosage forms of Chuanxiong Chatiao Preparations were different in chemical compositions and content.

Key words： Chuanxiong Chatiao preparations; dosage form; HS-SPME-GC-MS; volatile components

川芎茶调散是中医临床经典的方剂，此方始载于《和剂局方·卷之二治伤寒》，组方为川芎、荆芥、白芷、羌活、细辛、防风、薄荷、炙甘草; 2015年版《中国药典》[1]收载有川芎茶调散、川芎茶调丸、川芎茶调丸（浓缩丸）、川芎茶调袋泡茶（川芎茶调袋泡剂）、川芎茶调片和川芎茶调颗粒等不同剂型;其功能与主治均为疏风止痛，用以外感风邪所致的头痛，或有恶寒、发热、鼻塞。在近期的药理学研究[2-5]中发现川芎茶调散具有解热、镇痛、抗炎、耐缺氧、对帕金森多巴胺神经损伤保护等作用。目前对川芎茶调制剂的研究主要集中在临床和药理研究，尚未发现对川芎茶调制剂不同剂型的挥发性成分的分析。本试验首次应用HS-SPME-GC-MS技术对市面上川芎茶调制剂不同剂型的挥发性成分进行提取和比较，希望对川芎茶调制剂质量控制标准及功能主治方面有所帮助。……