张一帆 李星 张瑜

摘要 目的：观察松郁安神方对肝郁证失眠大鼠机体褪黑素水平的影响。方法：将40只大鼠随机分成正常对照组、模型组及松郁安神方低、中、高剂量组，采用慢性夹尾刺激和腹腔注射对氯苯丙氨酸（PCPA）复合因子造模法建立肝郁证失眠大鼠模型，给药后测定肝郁证失眠大鼠机体的脑脊液、血清、脑组织（下丘脑、海马）褪黑素（MT）水平的变化。结果：肝郁证失眠大鼠机体脑脊液、血清、脑组织（下丘脑、海马）的MT水平均显著低于正常组（P<0.05），松郁安神方低剂量组脑脊液、血清、海马的MT水平及松郁安神方中剂量组血清的MT水平显著高于模型组（P<0.05），而松郁安神方低剂量组血清的MT水平与松郁安神方中剂量组比较无显著差异（P>0.05）。结论：松郁安神方对肝郁证失眠大鼠具有促睡眠作用，其作用机制可能是通过提高机体褪黑素水平而实现。

关键词 松郁安神方;失眠;肝郁证;褪黑素

Abstract Objective：To observe the effect of Song Yu An Shen Fang on levels of melatonin in rats with insomnia with syndrome of stagnation of liver qi.Methods：A total of 40 rats were randomly divided into normal control group，model group，low，medium and high dose group of Song Yu An Shen Fang group.The insomnia rat model of liver depression syndrome was established by chronic tail clamping stimulation and intraperitoneal injection of PCPA compound factor.The changes of melatonin（MT）in cerebrospinal fluid，serum and brain tissue（hypothalamus and hippocampus）of insomnia rats with stagnation of liver qi syndrome were measured after administration.Results：The MT level of cerebrospinal fluid，serum and brain tissue（hypothalamus and hippocampus）in the insomnia rats with stagnation of liver qi syndrome was significantly lower than that in the normal group（P<0.05）.The MT levels of CSF，serum and hippocampus in the low dose group and the middle dose group were significantly higher than those in the model group（P<0.05）.There was no significant difference in MT level between the low dose group and the middle dose group（P>0.05），Conclusion：The effect of Song Yu An Shen Fang on promoting sleep of insomnia with syndrome of stagnation of liver qi may be achieved by increasing the level of melatonin.

Keywords Song Yu An Shen Fang; Insomnia; Syndrome of stagnation of liver qi; Melatonin

中圖分类号：R256.23文献标识码：Adoi：10.3969/j.issn.2095-7130.2020.03.074

中医在治疗失眠上有独有的优势，其病因病机虽复杂，但其发病与个体的体质和性格特征密切相关，临床上多数失眠的反复发作与异常情绪常相互伴随，并且一些针对失眠的证型和方药研究[1，2]显示从肝郁论治失眠具有较好疗效。

黄俊山教授结合多年失眠的临床经验，创制了松郁安神方（药用：甘松、郁金、玫瑰花、生龙骨、珍珠母、夜交藤、丹参、酸枣仁）主要针对肝郁证而设，功在疏肝解郁、安神定志，经临床证实疗效确切，但作用机制需要进一步明确。本文通过观察松郁安神方对肝郁证失眠大鼠在脑脊液、血清、脑组织（下丘脑、海马）中褪黑素（MT）水平的变化，探讨松郁安神方影响睡眠的药效作用机制。

1 材料与方法

1.1 动物与分组 健康雄性SD大鼠，8周龄，体质量180～200 g，购于上海斯莱克实验动物有限责任公司[动物合格证编号：20170005009597，许可证号码：SCXK（沪）2017-0005]。……