60CO一γ辐照对NaCl胁迫下藜麦种子萌发的影响
2019-08-03梁晓艳顾寅钰付娆张海洋衣葵花王向誉郭洪恩
梁晓艳 顾寅钰 付娆 张海洋 衣葵花 王向誉 郭洪恩



摘要:采用不同剂量(0-500 Gy)的60 Co -γ射线辐照藜麦种子,研究经不同剂量射线辐射处理的藜麦种子在不同浓度NaCl(O~400 mmol/L)胁迫下种子的萌发特性,以期筛选出能够提高藜麦种子耐盐性的最佳辐射剂量。结果表明:隨着盐浓度的升高,种子发芽率、发芽势和发芽指数呈逐渐下降趋势,高盐环境下下降较为显著;不同剂量的60Co-γ辐照处理对种子萌发的影响因盐浓度的不同而存在差异,即0-200 mmol/L NaCl条件下,种子发芽率及发芽时间随着辐照剂量的增加呈逐渐降低趋势,而300、400mmol/L NaCl条件下,种子发芽率随着辐照剂量的增加呈先升高后降低趋势,300 Gy是促进高盐环境下种子萌发的最佳辐照剂量。
关键词:藜麦;盐胁迫;60Co一γ辐照;种子萌发
Effects of 60Co—γ Irradiation on Seed Germination
of Quinoa under NaCI Stress
Liang Xiaoyan, Gu Yinyu, Fu Rao, Zhang Haiyang, Yi Kuihua, Wang Xiangyu, Guo Hong' en
Abstract
The effects of 60Co-γ irradiation with different doses (0~500 Gy) on the germination rate,germination potential and germination index of quinoa seeds under NaCl (0~400 mmol/L) stress were studiedin order to select the best irradiation dosage for improving salt tolerance of quinoa seeds. The results showedthat with the increase of salt concentration, the germination rate, germination potential and germination index ofseeds decreased gradually. The effect of 60Co- γ irradiation with different doses on seed germination was variedwith the different salt concentration. Under the condition of 0~200 mmol/L NaCl, the seed germination rateand germination time decreased gradually with the increase of irradiation dose, while the germination rate ofseeds increased firstly and then decreased with the increase of irradiation dose under 300 -400 mmol/L NaCl.In conclusion,300 Gy was the best irradiation dose to promote seed germination in high salinity environment.
Keywords
Quinoa; Salt stress; 60Co-γ irradiation; Seed germination
藜麦( Chenopodium quinoa Willd.)为苋科藜属一年生双子叶草本植物,原产于南美洲安第斯地区,距今已有7 000多年的栽培历史[1]。藜麦具有非常高的营养价值,其蛋白质含量在16%左右,高于水稻和玉米,与小麦相当,且人体必需氨基酸比例均衡,易于被人体吸收[2,3]。同时,藜麦富含维生素B、维生素C、维生素E和矿物质,以及皂苷、多糖、黄酮等生物活性物质[4,5]。除营养价值突出外,藜麦还具有耐寒、耐旱、耐贫瘠、耐盐碱等特性[6],尤其是对盐碱环境具有较强的适应性,有些藜麦品种可以耐受海水一样高的盐胁迫(电导率40dS/m,或相当于约400 mmol/LNaCl)[7]。因此,藜麦在盐碱地开发利用及优良作物筛选中具有广泛的应用前景和重要的开发利用价值。目前关于藜麦的研究多集中于不同地区的引种栽培及耐盐机理研究等方面,而关于盐碱地耐盐品种的选育研究相对较少,因此,筛选和培育适宜盐碱地种植的藜麦品种是盐碱地开发利用的重要途径。……
