王佳慧 郑洋 王嘉孺 李卫民

[摘要] 目的 运用DAVID数据库得到通路和基因的相关信息，分析出重要基因的功能和分布，筛选出与慢性萎缩性胃炎（CAG）相关的靶点基因，促进对CAG发病机制的研究以及新药的开发。 方法 2018年6月以“chronic atrophic gastritis”为关键词在NCBI数据库中检索CAG的相关基因，将得到的基因数据导入DAVID数据库，得到基因富集的通路数据。15个非冗余基因富集在20条KEGG通路上进行了分析，将15个基因输入String数据库做相互作用的网络图，将富集在通路上的关键基因也做网络图，并且将这两个网络图进行比较。 结果 在两张网络图中，FASLG、FAS、ICAM1、IL1B、AKT1、STAT3、TGFβ1、IL4R、IL11、IL23A是位于关系度排名靠前的基因。 结论 STAT3、ICAM1、IL1B、TGFβ1和AKT1五个基因与CAG相关，需要进一步深入研究，通过分析与疾病相关基因的所在通路和基因之间互作的网络图，有利于了解疾病的发病机制，并为新药研发提供可靠的靶点。

[关键词] 慢性萎缩性胃炎;基因互作网络;KEGG通路;富集分析

[中图分类号] R573.32          [文献标识码] A          [文章编号] 1673-7210（2019）01（b）-0159-06

[Abstract] Objective By means of DAVID database， the information related to pathway and gene was obtained， the function and distribution of important gene was analyzed， the target gene related to chronic atrophic gastritis （CAG） was screened， so as to promote the research on CAG pathogenesis and the development of new drug. Methods By taking “chronic atrophic gastritis” as key words， the gene related to CAG was retrieved in NCBI database in June 2018 and the obtained gene data was imported into DAVID database， then the pathway of gene enrichment was figured out. Next， 15 pieces of non-redundant gene enrichment were analyzed on 20 KEGG pathways. Besides， 15 genes were input into String database to draw interaction network diagram， another network diagram for the key genes of enrichment on pathway was also made， the two network diagrams were compared. Results In the two network diagrams， the top-ranked genes in terms of relational hierarchy turned out to be FASLG， FAS， ICAM1， IL1B， AKT1， STAT3， TGFβ1， IL4R， IL11 and IL23A， etc. Conclusion The five genes of STAT3， ICAM1， IL1B， TGFβ1 and AKT1 have something to do with CAG and an in-depth research should be conducted. Moreover， the diagram which shows the interaction between the pathway and the genes related to CAG should be analyzed， which can do good to the understanding on the pathogenesis of CAG and can provide reliable target for the development of new drug.

[Key words] Chronic atrophic gastritis; Gene interaction network; KEGG pathway; Enrichment analysis

慢性萎縮性胃炎（chronic atrophic gastritis，CAG）是发生在中老年人群中的常见消化系统疾病，是胃癌早期病变的必经过程[1]。中国CAG患者发病率居亚洲首位。该病发病缓慢，治疗周期长，西医治疗不能根除病因易反复，严重影响我国居民的生活质量，如何防治CAG是目前社会关注的热点问题[2-3]。CAG和胃癌密切相关，临床上把CAG称为胃癌前病变，是我国慢性消化道疾病需要突破的重要难点[4-5]。CAG的发病机制尚未明确，目前尚无特效治疗药物。本研究结合KEGG通路和基因互作网络进行整体分析，寻找CAG的药物靶点。……