外源激素对盐胁迫下烟草幼苗生理抗性的影响
2018-12-08李武琴曾乙心陈光勇丁金玲马园正李建林兰建强
李武琴 曾乙心 陈光勇 丁金玲 马园正 李建林 兰建强
摘要:用不同浓度的α-NAA、GA3、ABA 喷施处理100 mmol/L NaCl 胁迫下的烟草幼苗,测定烟草幼苗丙二醛含量和超氧化物歧化酶、过氧化物酶、多酚氧化酶活性等指标,探究外源激素对盐胁迫下烟草幼苗生理特性的影响。结果表明:100 mmol/L NaCl胁迫下,烟草幼苗MDA含量和SOD、POD、PPO活性升高;喷施外源激素α-NAA、GA3、ABA可以降低NaCl胁迫下烟草幼苗MDA含量和SOD、POD、PPO活性,减轻NaCl对烟苗叶片膜脂过氧化的程度,增强烟苗对盐胁迫的抗性。因此,外源激素α-NAA、GA3、ABA可缓解NaCl对烟草幼苗胁迫,其中喷施ABA及50 mg/L α-NAA、GA3缓解效果较好。
关键词:烟草;盐胁迫;外源激素;生理抗性
中图分类号:S572 文献标识号:A 文章编号:1001-4942(2018)09-0036-04
Abstract Tobacco seedlings under salt stress were treated by spraying different concentrations of α-NAA, GA3 and ABA, and then the malondialdehyde (MDA) content, and the activity of superoxide dismutase (SOD), peroxidase (POD) and polyphenol oxidase (PPO) of tobacco seedlings were measured to study the influence of exogenous hormones on tobacco seedlings physiological characteristics under salt stress. The results showed that MDA content, and SOD, PPO and POD activities of tobacco seedlings increased under 100 mmol/L NaCl stress. Spraying exogenous hormone such as such as α-NAA, GA3 and ABA could reduce the MDA content, and SOD, POD and PPO activities of tobacco seedlings under NaCl stress and reduced the degree of NaCl to the membrane lipid peroxidation of tobacco leaves, it could also enhance the resistance to salt stress. Therefore, exogenous hormones including α-NAA, GA3 and ABA could alleviate NaCl stress on tobacco seedlings, among which exogenous ABA and 50 mg/L α-NAA and GA3 had the better effect on remission effect.
Keywords Tobacco; Salt stress; Exogenous hormones; Physiological resistance
鹽胁迫对植物的危害主要是渗透胁迫、离子毒害、离子不平衡以及营养缺乏[1,2]。一方面土壤中可溶性盐增加导致土壤溶液渗透压增高、水势降低,植物从土壤中吸收水分困难、出现生理性干旱;此外,一些盐分离子对植物具有直接毒害作用[3]。另一方面,溶液中过量的盐离子会阻碍植物对以 K+ 为主的一些必需矿质元素的吸收,造成营养离子亏缺,同时植物细胞中过量的 Cl- 和 Na+ 也会影响细胞内酶的活性,细胞的代谢和生理功能受到不同程度的破坏[4]。高盐胁迫通常导致植物生长发育周期缩短,生物量和经济产量下降,是制约植物生长和发育的主要因素之一 [1]。
烟草是一种对氯离子较为敏感的作物,其生长发育要求土壤氯离子含量低于0.6%,因此,土壤盐渍化对烟草生产具有较大的限制作用。李莉等[5]研究表明,NaCl浓度达到100 mmoL/L时,烟草幼苗生长及生理特性明显受到胁迫。……
