楼彩霞 徐阎 周王谊

【摘 要】 目的： 通过大鼠体重、体重增重、摄食量、食物利用率和消化酶测定实验，以及小鼠小肠运动实验来评价低咖啡因普洱茶提取物促进动物消化功能。方法： 通过优化普洱茶提取工艺得到低咖啡因普洱茶提取物。依据保健食品促进消化功能评价方法设计大、小鼠实验，实验结束时测定大鼠的体重、体重增重、摄食量、食物利用率、1h胃液量、胃蛋白酶活性和排出量，以及小鼠的墨汁推进率。结果： 与对照组相比，普洱茶提取物高剂量组大鼠的1h胃液量和胃蛋白酶排出量增加（P<0.05，P<0.01）；中、高剂量组小鼠的墨汁推进率转换值升高（P<0.01）。结论： 此低咖啡因普洱茶提取物具有促进动物消化的功能，具有一定的开发价值。

【关键词】 低咖啡因普洱茶提取物；促进消化功能；大鼠；小鼠

【中图分类号】R285.5 【文献标志码】 A 【文章编号】1007-8517（2018）14-0019-04

Abstract：Objective To objectively appraise the promoting digestion function of low-caffeine Pu-erh Tea extract through rat tests for body weight， weight gain， food intake， digestive enzymes， food utilization rate and mouse test for small intestinal movement. Methods The low-caffeine Pu-erh Tea extracts were obtained by optimize the Pu-erh Tea's extraction process. We designed experiments according to the evaluation method of promoting digestion on health food. We measured the body weight， weight gain， food intake， digestive enzymes， food utilization rate in rat tests and ink propelling rates in mouse small intestinal movement test respectively. Results The rat tests showed that the gastric juice within one hour and gastric pepsin output of low-caffeine Pu-erh Tea extract group with high dose were increased compared with the control group （P<0.05， P<0.01）. The mouse test showed that the ink propelling rates of low-caffeine Pu-erh Tea extract groups with middle and high dose were increased compared with the model control group （P<0.01）. Conclusions This study suggests that the low-caffeine Pu-erh Tea extract can promote digestion in animals， have certain development application value.

Keywords：Low-caffeine Pu-erh Tea Extracts；Promote Digestion；Rats；Mice

功能性消化不良是常見的胃肠道疾病，临床出现餐后饱胀感、上腹部疼痛或燃烧等症状，影响人们生活质量，症状较轻时容易被忽视[1]。功能性消化不良的病理生理学尚不完全清楚，常被认为是上消化道炎症和运动障碍[1]。普洱茶，为山茶科植物普洱茶的嫩叶，味苦、甘，性寒，归胃、肝、大肠经。普洱茶作为一种传统历史名茶，因其独特的口感和多种保健功能深受人们喜爱。《本草纲目拾遗》记载，普洱茶绿色者更佳，消食化痰，清胃生津，功力尤大[2]。现代文献记载，普洱茶及其提取物具有抗肿瘤[3]、保护神经系统[4]、降脂减肥[5]、改善卵巢切除术后的骨质疏松[6]，以及杀菌、抗疲劳、抗氧化和预防心血管疾病等[7]功能。

本实验的普洱茶产地是云南普洱，通过优化普洱茶的提取工艺得到低咖啡因普洱茶提取物，主要成分有茶多酚、茶褐素和茶多糖等。……