张萌 高博 李勇辉 杨庆雄

【摘 要】 目的：评价青阳参总苷（QYS）的抗抑郁活性。方法：60只大鼠分为对照组、模型组、QYS低中高剂量组，氟西汀作为阳性对照组。首先进行10d社会挫败应激，第5天开始灌胃给药，然后测试糖水偏爱、旷场、惊跳反射和前脉冲抑制等行为。结果：与模型组相比，QYS和氟西汀能显著抑制应激8 d的糖水偏爱下降，旷场试验大鼠自主活动性和不动时间没有显著变化，而QYS高剂量组和阳性对照组能增加大鼠僵直时间；大鼠惊跳反射和前脉冲抑制没有显著变化，而QYS高剂量组能显著降低大鼠105dB惊跳反射幅值；与模型组间的差异有统计学意义（P<0.05）。结论：QYS对社会挫败应激大鼠抑郁行为具有调节作用。

【关键词】 青阳参总苷；社会挫败应激；抗抑郁

【中图分类号】R285.5 【文献标志码】 A【文章编号】1007-8517（2018）15-0017-04

Abstract：Objective To evaluate the antidepressant activity of Qingyangshen glycosides （QYS）. Methods 60 rats were divided into control group， model group and QYS treatment group， and fluoxetine wasused as positive control group. Firstly， rats were stressed 10 days by a social defeat stress program， and drugs were administered intragastrically on the 5th day of the stress. Thebehavioral effectswere tested using syruppreference， open field， startle reflexand prepulse inhibitiontests. Results QYS and fluoxetine could significantly inhibit the decrease of syrup preference value induced by stress， and no significant change was observed in the autonomic activity and immobility of rats. However， the freezing time was increased in high dose of QYS and positive control groups in the open field tests.Nodifference was observed between startle reflex and prepulseinhibition in rats after stress， but the startle reflex amplitude of rats on 105 dB was significantly inhibited in the high dose of QYS group， being comparing with the stress group. And the difference was statistically significant between the model group（P<0.05）.Conclusion QYS showed an antidepressanteffect onsocial defeat stress rats.

Keywords：Qingyangshen Glycosides； Social Defeat Stress； Antidepressant

随着当今社会压力上升，焦虑症、抑郁症等慢性应激相关的精神障碍受到研究者的特别关注。抑郁症是一种慢性的反复发作的精神疾病，临床核心症状表现为快感缺失，患者甚至产生自杀倾向，社会危害性很大。由于抑郁症的病理机制较为复杂，所以不同作用机制的药物对抑郁症的治疗效果也不一样[1-3]，故从不同作用机制出发，开发抗抑郁药物，具有重要意义。

青阳参（Cynanchum otophyllum）为萝藦科鹅绒藤属植物，是西南地区的重要的民族药之一，具有抗癫痫[4-5]、抗抑郁[6]等药理活性作用，研究发现青阳参的抗抑郁活性成分为C21甾体苷类[7-8]。青阳参总苷对强迫游泳和悬尾等绝望筛选模型[6]和慢性不可预知温和应激模型动物[9]的抑郁具有显著的调节作用。……