黄博 金国荣 屈重霄 马海宁 丁彩云

摘 要 目的：检测了Notch信号通路中各受体及配体在胃癌中的表达，探讨Notch信号通路在胃癌发生发展中的作用。方法：应用实时荧光定量PCR法检测了NOTCH1/2/3/4、JAG1/2、DLL1/3/4在12 例正常胃黏膜组织中的表达情况及在胃癌组织中的异常表达情况；应用免疫印迹法检测了NOTCH1在对应胃癌/癌旁组织中的表达。结果：在正常组织中受体NOTCH2和配体JAG1的表达水平较高，其余表达水平较低；在胃癌组织中，NOTCH1/3/4和JAG2的表达水平明显提高，而其他受体和配体的表达水平变化不明显。NOTCH1蛋白在胃癌组织中的表达较癌旁组织明显增高。结论：Notch信号通路中部分受体及配体在胃癌组织中表达明显提高，Notch信號通路参与了胃癌的发生发展。

关键词 胃癌 Notch信号通路 受体 配体

中图分类号：R735.2； R730.231 文献标志码：A 文章编号：1006-1533（2018）15-0057-05

Expressions of the receptors and ligands in Notch signaling pathway in gastric cancer tissues*

HUANG Bo1**， JIN Guorong2， QU Chongxiao3， MA Haining1， DING Caiyun2

（1. Department of General Surgery， 2. Central Laboratory， 3. Department of Pathology， Shanxi Provincial Peoples Hospital， Taiyuan 030012， China）

ABSTRACT Objective： To detect the expressions of receptors and ligands in Notch signaling pathway in gastric cancer tissues and to explore the role of Notch signaling pathway in the occurrence and development of gastric cancer. Methods： The expression levels of NOTCH1/2/3/4， JAG1/2 and DLL1/3/4 in normal gastric tissues and the aberrant expression levels in gastric cancer tissues were studied by real-time RT-PCR. The expression of NOTCH1 protein in gastric cancer or adjacent non-tumor tissues was detected using Western Blotting. Results： The expressions of NOTCH2 and JAG1 were relatively high in normal gastric tissues while the others were relatively low. The expressions of NOTCH1/3/4 and JAG2 were significantly increased in gastric cancer tissues as compared with normal gastric tissues while other receptors and ligands showed little differences. The expression of NOTCH1 protein was significantly higher in gastric cancer tissues than adjacent non-tumor tissues. Conclusion： The expressions of a part of receptors and ligands in Notch signaling pathway are significantly increased in gastric cancer tissues， showing that Notch signaling pathway is involved in the occurrence and development of gastric cancer.

KEY WORDS gastric cancer； Notch signaling； receptor； ligand

胃癌是一种发生于胃黏膜的恶性肿瘤，是消化道常见的癌症之一，其病死率居常见肿瘤病死率的第3位[1]。世界胃癌患者中有超过70%来自发展中国家，其中中国的胃癌发生率最高[2]。目前外科手术是胃癌治疗的主要方法，随着化疗、放疗、免疫治疗的进步，以及以手术为主的多学科团队（MDT）的综合治疗的发展，胃癌的治疗取得了一定的进步，但并没有从根本上改变胃癌高死亡率的现状，其总体生存率和无瘤生存率仍然很低。因此，迫切需要对胃癌进行更深入的研究，寻找治疗胃癌更灵敏的分子生物标记物，为胃癌的诊断和治疗提供新的突破口。

Notch信号通路在进化上保守，参与许多细胞进程包括增殖、分化、凋亡和干细胞维持等[3]。……