臧福才 刘明月 刘敏 唐伟

[摘要] 目的 探討不同行为特征肺癌小鼠血管内皮生长因子（VEGF）的表达及自然杀伤细胞（NK）的活性差异。 方法 Balb/c小鼠80只，雄性，进行旷场试验，筛选高活跃组和低活跃组小鼠，每组各20只；取处于对数生长期的LLC小鼠肺癌细胞，接种于高活跃组和低活跃组小鼠右腋皮下，建立小鼠肺癌模型。两组相同条件饲养14 d后，MMT法检测两组小鼠脾脏NK细胞的杀伤活性；游标卡尺测量肿瘤的长、短径，计算肿瘤体积，电子天平称肿瘤重量；免疫组织化学法检测两组小鼠肿瘤组织VEGF蛋白的表达情况。 结果 高活跃组小鼠NK细胞的杀伤活性为（19.73±3.55）%，高于低活跃组[（16.47±3.29）%]，差异有统计学意义（P < 0.05）。高活跃组小鼠肿瘤体积为（0.78±0.14）cm3，低于低活跃组[（0.94±0.24）cm3]，差异有统计学意义（P < 0.05）。高活跃组小鼠肿瘤重量为（0.84±0.19）g，低于低活跃组[（1.00±0.22）g]，差异有统计学意义（P < 0.05）。高活跃组小鼠VEGF阳性表达率为85.0%，明显低于低活跃组（95.0%），差异有统计学意义（P < 0.05）。 结论 不同活跃程度荷瘤小鼠肺癌组织生长速度不同，可能与小鼠活跃程度影响NK细胞活性、肿瘤组织VEGF蛋白表达有关。

[关键词] 行为特征；肺癌；NK细胞；血管内皮生长因子

[中图分类号] R734.2 [文献标识码] A [文章编号] 1673-7210（2018）05（c）-0018-04

Study on the differences of the expression of vascular endothelial growth factor and the activity of NK cells in lung cancer mice with different behavior characteristics

ZANG Fucai1 LIU Mingyue2 LIU Min1 TANG Wei1

1.Neurology Department， Xinhua Hospital Affiliated to Dalian University， Liaoning Province， Dalian 116021， China； 2.the Second Radiotherapy Department， Handan Central Hospital， Hebei Province， Handan 056000， China

[Abstract] Objective To investigate the differences of the expression of vascular endothelial growth factor （VEGF） and the activity of NK cells in lung cancer mice with different behavior characteristics. Methods Eighty male Balb/c mice were taken open field test to screen high active group and low active group， with 20 mice in each group. The logarithmic phase LLC cells were inoculated in the right axillary subcutaneous of high active group and low active group to establish lung cancer model. After 14 days under the same feeding condition， the killing activity of spleen NK cells in the two groups was detected by MMT method. The long and short diameters of tumor were detected by vernier caliper， the tumor volume was calculated， and the tumor weight was measured by electronic scales. Immunohistochemistry method was used to detect the expression of VEGF in the two groups. Results The killing activity of NK cells in the high active group was （19.73±3.55）%， which was significantly higher than that of low active group [（16.47±3.29）%]， the difference was statistically significant （P < 0.05）. The tumor volume of high active group was （0.78±0.14） cm3， which was smaller than that of low active group [（0.94±0.24） cm3]， the difference was statistically significant （P < 0.05）. The tumor weight of high active group was （0.84±0.19） g， which was lower than that of low active group [（1.00±0.22） g]， the difference was statistically significant （P < 0.05）. The positive expression rate of VEGF in the high active group was 85.0%， which was lower than that of low active group （95.0%）， there was statistically significant difference （P < 0.05）. Conclusion Different activity degrees of tumor-bearing mice have different growth rates of lung cancer tissue， which may be connected with the activity of NK cells and the expression of VEGF protein in tumor tissue.

[Key words] Behavior characteristics； Lung cancer； NK cells； Vascular endothelial growth factor

癌症是目前对人类健康威胁最大的疾病之一[1]。癌症也是一种较为明确的身心疾病，个性行为特征和精神心理因素与癌症的发生、发展有着非常密切的关系。英国学者Greer等结合自己的研究结果，总结了癌症患者的人格特征：压抑自己，不善于将内心的感受和不良情绪外泄，对生活事件所产生的负性情绪，压抑到内心世界[2]。处在开放环境中的动物实验的特征与人存在相似之处。Dellu等[3]认为高活跃程度的大鼠行为与性格开朗、积极探索的人行为相似，低活跃程度的大鼠与消极态度、高度恐惧感和害羞感特征的人相似。

自然杀伤细胞（NK）既是机体内的自然防御细胞，又兼有重要的免疫调节功能[4]。肿瘤血供丰富，其生长离不开血管的生成，而血管内皮生长因子（VEGF）起关键作用[5]。……