刘庆滨 陈艳昕 肖芳 周颖 张辉

[摘要] 目的 分析转移抑制因子1（MTSS-1）及胚胎干细胞关键蛋白（SOX2）在结肠癌组中表达及与临床病理关系。 方法 选取2016年1月～2017年6月秦皇岛市第一医院收治的80例结肠癌患者作为研究对象，采用二步法免疫组织化学方法检测患者的结肠癌组织中MTSS1及SOX2表达水平，并分析临床病理特征与MTSS1及SOX2表达的相关性。 结果 结肠癌组织中MTSS1及SOX2蛋白阳性表达率均显著低于癌旁毗连区，差异有统计学意义（P < 0.01）；不同分化程度、淋巴结转移、Dukes分期患者肿瘤组织中MTSS1及SOX2阳性表达率差异有统计学意义（P < 0.01）；分化程度是MTSS1阳性表达的危险性因素（P < 0.05），分化程度及淋巴结转移是SOX2阳性表达的危险性因素（P < 0.05）。 结论 结肠癌组织MTSS1与SOX2在结肠癌组织中的表达与分化程度密切相关，且淋巴结转移与SOX2表达显著相关。

[关键词] 结肠癌；MTSS1；SOX2；临床病理

[中图分类号] R574.62 [文献标识码] A [文章编号] 1673-7210（2018）05（b）-0080-03

Expression of MTSS1 and SOX2 in colon cancer tissue and its relationship with clinicopathological characteristics

LIU Qingbin1 CHEN Yanxin2 XIAO Fang2 ZHOU Ying2 ZHANG Hui2

1.Department of General Surgery， Qinhuangdao City No.1 Hospital， Hebei Province， Qinhuangdao 066000， China；2.Department of Pathology， Qinhuangdao City No.1 Hospital， Hebei Province， Qinhuangdao 066000， China

[Abstracts] Objective To analyze the relationship between the expression of MTSS-1 and SOX2 and clinicopathological in colon cancer. Methods From January 2016 to June 2017， 80 cases colon cancer patients， who were treated in Qinhuangdao City No.1 Hospital were selected as the research object， the levels of MTSS1 and SOX2 expression were tested by two step immunohistochemical method， and the clinical and pathological features of MTSS1 and SOX2 expression correlation were analyzed. Results The positive expressions of MTSS1 and SOX2 protein in colon cancer tissues were significantly lower than the adjacent contiguous zone， the differences were statistically significant （P < 0.01）. The positive expressions of MTSS1 and SOX2 were significant difference between the deposit rate of different degree of differentiation， lymph node metastasis and Dukes staging of cancer tissue， the differences were statistically significant（P < 0.01）. The degree of differentiation was the risk factor of MTSS1 positive expression （P < 0.05）. The degree of differentiation and lymph node metastasis were risk factors of positive expression of SOX2 （P < 0.05）. Conclusion The expression of MTSS1 and SOX2 in colon cancer tissues is closely related to the degree of differentiation， and the lymph node metastasis is significantly related to the expression of SOX2.

[Key words] Colon cancer； MTSS1； SOX2； Clinicopathological

結肠癌是现阶段临床中较为常见的恶性肿瘤之一，其恶性肿瘤的转移及复发与患者死亡密切相关[1]。目前随着人们生活习惯的改变，我国结肠癌发生率呈逐年递增趋势，严重威胁患者健康安全[2]。有学者采用mRNA差异技术对膀胱癌细胞系进行分析发现，转移抑制因子1（metastasis suppressor 1，MTSS-1）在恶性肿瘤转移及复发过程中起到十分重要的作用，是目前肿瘤转移研究新热点[3-4]。MTSS1在细胞伪足迹细胞骨架组装密切相关，是候选肿瘤转移抑制基因[5]。胚胎干细胞关键蛋白（SOX2）是重要的转录因子家族成员，有研究结果显示，SOX2蛋白在器官早期发育过程中起到十分重要的作用，是哺乳动物胚胎发育的必要因子[6-7]。此外，SOX2具有极高的表达调控敏感性，微弱变化均可能导致细胞分化，且在肿瘤的发生、发展过程中均起到十分重要的作用[8]。但MTSS-1及SOX2在结肠癌发生及发展过程中仍鲜有报道，因而笔者对秦皇岛市第一医院（以下简称“我院”）收治的结肠癌患者作为研究对象，分析MTSS-1及SOX2在结肠癌组中表达及与临床病理关系。……