梁法清 杜正贵 何阳君

[摘要] 目的 探討乳腺癌患者癌组织及腋窝淋巴结中乙醛脱氢酶1（ALDH1）以及乳腺癌耐药蛋白（BCRP）的表达状况与乳腺癌病理特征的关系。 方法 选取2013年1月～2016年3月在四川大学华西医院治疗的乳腺癌患者共84例，同时选取距肿瘤边缘大于1 cm正常乳腺组织（癌旁正常组织）作为对照，采用免疫组化法检测ALDH1和BCRP蛋白的表达。 结果 乳腺癌组织中ALDH1和BCRP蛋白表达率明显高于癌旁正常组织（P < 0.01）；腋窝淋巴结有转移患者癌组织中ALDH1和BCRP蛋白表达阳性率明显高于无腋窝淋巴结转移患者（P < 0.05）；雌激素受体（ER）阳性患者乳腺癌组织中BCRP蛋白表达阳性率明显高于ER阴性患者（P < 0.05）；而乳腺癌组织中ALDH1和BCRP蛋白表达与患者年龄、绝经情况、肿瘤大小、组织分级、TNM分期和孕激素受体（PR）均无相关性（P > 0.05）；乳腺癌患者ALDH1和BCRP蛋白的表达也无相关性（rs=-0.092，P > 0.05）。 结论 ALDH1和BCRP蛋白在乳腺癌发生发展中有一定作用，可能参与了肿瘤的侵袭、转移等过程。

[关键词] 乙醛脱氢酶1；乳腺癌耐药蛋白；乳腺癌；病理特征

[中图分类号] R737.9 [文献标识码] A [文章编号] 1673-7210（2018）05（b）-0076-04

The relationship between the expressions of aldehyde dehydrogenase 1， breast cancer resistance protein in breast cancer tissues and pathological features of breast cancer

LIANG Faqing1 DU Zhenggui1 HE Yangjun2

1.Department of Breast Surgery， West China Hospital of Sichuan University， Sichuan Province， Chengdu 610041， China； 2.Department of Surgery， Chengdu Seventh People′s Hospital， Sichuan Province， Chengdu 610041， China

[Abstract] Objective To investigate the relationship between aldehyde dehydrogenase 1 （ALDH1）， breast cancer resistance protein （BCRP） expressions in tumor tissues and pathological features of breast cancer. Methods From January 2013 to March 2016， 84 patients with breast cancer in West China Hospital of Sichuan University and the normal breast tissues （adjacent tissues） which was used for control group is defined as the edge of cancer tissueedge greater than 1 cm were selected. The expression of ALDH1 and BCRP protein was detected by immunohistochemistry. Results The expression rates of ALDH1 and BCRP protein in breast cancer tissues were significantly higher than those in adjacent tissues （P < 0.01）. The positive rates of ALDH1 and BCRP protein expression in patients with axillary lymph node metastasis were significantly higher than those in patients without axillary lymph node metastasis （P < 0.05）. The positive rate of BCRP protein expression in estrogen receptor （ER） positive patients was significantly higher than that in ER negative patients （P < 0.05）. The expression of ALDH1 and BCRP protein was not correlated with age， menopause， tumor size， histological grade， TNM stage and progesterone receptor （PR） （P > 0.05）. There was no correlation between the expression of ALDH1 and BCRP protein in breast cancer （rs=-0.092， P > 0.05）. Conclusion ALDH1 and BCRP may play an important role in the development of breast cancer， which may be involved in the tumor invasion， metastasis etc.

[Key words] Aldehyde dehydrogenase 1； Breast cancer resistance protein； Breast cancer； Pathological characteristics

乳腺癌是临床上较为常见的恶性肿瘤，且近年来呈现出了明显的上升趋势，美国癌症协会报道中乳腺癌在女性新发病历中居于首位[1]。由此可见乳腺癌在人群中的疾病负担较重，因此对乳腺癌的发病机制的研究极为重要，是我们对乳腺癌预防、诊断、治疗的基石。对于乳腺癌发病及侵袭机制的研究，可以为临床上乳腺癌患者的靶向治疗提供新的靶点，并可以为乳腺癌患者的临床预后评估提供可靠的实验室指标。我们知道，一些肿瘤生物学指标可以促进癌细胞的增殖与分化，进而参与到癌症的发生、发展及侵袭转移等过程。有基础研究报道，乙醛脱氢酶1（aldehyde dehydrogenase 1，ALDH1）以及乳腺癌耐药蛋白（breast cancer resistance protein，BCRP）可以通过诱导癌细胞细胞周期调控异常，提高癌组织对于抗癌药物的耐受能力，进而促进乳腺癌的发生发展[2-4]。然……