李定银 王燕 罗群 韦治艳 王召 杨洪 金道超

摘 要：為探讨毒死蜱（Chlorpyrifos）对褐飞虱Nilaparvata lugens（Stl）的亚致死效应，本文采用稻茎浸渍法，分别以LC10和LC25浓度的毒死蜱胁迫褐飞虱3龄若虫，观察受胁迫后试虫发育历期、存活数、产卵量和雌虫寿命的变化情况，并组建经LC10、LC25浓度处理后F1代种群生命表，为褐飞虱的综合防治提供数据参考。结果表明：以毒死蜱LC10和LC25浓度分别处理褐飞虱3龄若虫，其F0代的孵化率与对照相比明显下降；处理组褐飞虱的种群相对适合度分别仅为0.86 和0.74，表明亚致死浓度毒死蜱可有效抑制褐飞虱种群的增长；通过组建F1代生命表发现，亚致死浓度处理褐飞虱后，能够显著抑制孵化率。另外，处理后褐飞虱的存活率、羽化率、孵化率与对照组相比均有所降低，对褐飞虱的发育历期有轻度延长。根据研究结果推测，将毒死蜱用于防治水稻害虫时，毒死蜱不但对褐飞虱有致死作用，其亚致死浓度胁迫能进一步抑制下一代褐飞虱的种群增长。

关键词：毒死蜱；褐飞虱；亚致死效应；生命表

中图分类号：S435.112.3

文献标识码：A

文章编号：1008-0457（2018）03-00 -0 国际DOI编码：10.15958/j.cnki.sdnyswxb.2018.03.001

Research of the sublethal effect of chlorpyrifos on Nilaparvata lugens （St

？倞 l） [Hemiptera： Delphacidae]

LI Ding-yin1，WANG Yan1，LUO Qun1，WEI Zhi-yan1，WANG Zhao1，3，YANG Hong1，2，JIN Dao-chao1*

（1. Guizhou Key Laboratory for Agricultural Pest Management of Mountainous Region， Institute of Entomology， Guizhou University， Guiyang 550025， China； 2. College of Tobacco Science of Guizhou University， Guiyang 550025， Guizhou Province， China； 3. School of Environment and Life Sciences， Kaili College， Kaili 556011， China）

Abstract：To investigate the sublethal effect of chlorpyrifos on Nilaparvata lugensus （St

？倞 l）， the rice stem dipping method was used in this study and the N. lugensus 3rd instar nymph was treated with the sublethal chlorpyrifos concentrations. Then the change of developmental period， fecundity and female longevity were studied. The time specific life table of F1 generation population was established. The results showed that the hatching rate of the F0 generation of the treatment group was significantly lower than that of the control group. The relative fitness of N. lugens in the treatment group was only 0.86 and 0.74， indicating that the sublethal concentration of chlorpyrifos could effectively inhibit the growth of the population of N. lugens.Through the establishment of the F1 generation life table， we were able to find that exposure to LC10 and LC25 could significantly inhibit the hatching rate of N. lugens. In addition， the survival rate， emergence rate and hatchability of N. lugens after treatments were lower than those of control group. The developmental period of N. lugens was slightly prolonged. The results suggested that chlorpyrifos could not only kill N. lugens but also further inhibit the population growth of the next generation of N. lugens when using it to control rice pests.

Key words：Chlorpyrifos； Nilaparvata lugens （St

l）； sublethal effect； life table

褐飞虱Nilaparvata lugens（Stl），属半翅目Hemiptera、飞虱科Delphacidae，是我国最重要的水稻害虫之一。其成虫和若虫均集中在水稻茎秆或穗基部取食，造成稻株萎焉，严重时整株枯死，成虫还可用产卵器刺破叶鞘产卵造成直接为害[1]，此外，褐飞虱还能传播水稻草矮病毒（Rice grassy stunt virus，GSV）、水稻齿叶矮缩病毒（Rice ragged stunt virus，RRSV）等病害，对水稻造成间接为害[2]，对水稻危害性极大，已成为最有毁灭性的水稻害虫，是我国以及许多亚洲国家当前水稻上的首要害虫[3]。……