施满容

摘要：以金线莲试管苗为材料，MS+6-BA 3.0 mg/L+NAA 0.3 mg/L+琼脂7 g/L+蔗糖25 g/L为基础培养基，采用SPSS正交设计试验，研究不同添加物、不同瓶子规格、不同光质等对金线莲试管苗生长过程中多糖含量积累的影响。结果表明，影响金线莲多糖含量的顺序为光质>添加物>瓶子规格。添加物中香蕉泥的均值最大为0.291，并且香蕉泥与苹果汁和胡萝卜汁之间具有显著性差异（P<0.05）。光质中蓝光的均值最大为0.289，并且蓝光与白光和红光之间具有显著性差异（P<0.05）。瓶子规格中6.5 cm的均值最大为0.279，与8.0 cm瓶子对比有显著性差异（P<0.05），与9.0 cm瓶子对比没有显著性差异（P>0.05）。优化后金线莲多糖含量累积的培养条件为MS+6-BA 3.0 mg/L+NAA 0.3 mg/L+琼脂7 g/L+蔗糖25 g/L+香蕉泥（pH 5.8）50 g/L+6.5 cm培养瓶+蓝光。

关键词：金线莲；组织培养；多糖含量

中图分类号：S567 文献标识码：A 文章编号：0439-8114（2018）04-0113-04

DOI：10.14088/j.cnki.issn0439-8114.2018.04.029

Effects of Different Culture Conditions on Polysaccharide Contents

in Anoectochilus roxburghii

SHI Man-rong

（Ningde Vocational and Technical College，Fuan 355000，Fujian，China）

Abstract： The in vitro plantlets were used as the materials，and MS+3.0 mg/L 6-BA+0.3 mg/L NAA+7 g/L agar+25 g/L glucose was used as the basal medium for studying the effects of organic additives， bottle sizes and light qualities on the accumulation of polysaccharide content during the growth of in vitro plantlets by the L9（34） orthogonal experimental design during in Anoectochilus roxburghii. The results showed that the order of polysaccharide content was light quality>additive>bottle size. The average value of banana mud in the additive was 0.291，and there was a significant difference between banana mud and apple juice and carrot juice（P<0.05）. The mean value of blue light in the light mass is 0.289， and there is a significant difference between blue light and white and red light （P<0.05）. The mean value of 6.5 cm in the bottle size was 0.279，which was significantly different from that of the 8.0 cm bottle（P<0.05），and there was no significant difference（P>0.05）. Consolidated results showed that the best culture conditions for the accumulation of polysaccharide content was MS+6-BA 3.0 mg/L+NAA 0.3 mg/L+7 g/L agar+25 g/L glucose+50 g/L banana（pH 5.8） in the culture bottle with 6.5 cm diameter in the blue light.

Key words： Anoectochilus roxburghii； tissue culture； polysaccharide contents

金線兰[Anoectochilus roxburghii（Wall.）Lindl.]俗称金线莲，为单子叶植物纲兰科开唇兰属植物，在中国福建、台湾等省和东南亚地区被视为珍稀名贵药材，享有“药王”“金草”“鸟人参”“神药”等美称[1]。金线莲中含有丰富的多糖，是其药用成分之一，具有抗肿瘤、抗氧化、抗衰老、增强免疫功能等作用[2]，是一种天然抗氧化剂。福建省金线莲产业规模持续快速扩大，已经成为福建省特色中草药产业之一。市场上的金线莲产品多数是采用组织培养技术进行种苗生产，由于组织培养条件和后期大田种植管理的差异，对金线莲有效成分积累产生很大的影响。目前，市场上还没有形成一套完整的适用于金线莲生产的质量标准，难以对金线莲有效药用成分和品质进行控制，导致市场上的金线莲产品参差不齐。

金线莲多糖含量是金线莲产品质量标准的重要控制成分，不同培养条件会影响金线莲多糖含量变化。如黄……