倪光

[摘要] 目的 更进一步对NSE及IGF-1在癫痫发作患儿血清中的临床表达及其临床意义进行探究。方法 方便择取2015年7月—2017年7月于该院儿科接受治疗的120例癫痫发作患儿进行回顾分析，设为观察组，另取120名健康儿童作为对照研究，设为对照组。对两组儿童均行ELISA及放射免疫分析法进行探究，对比两组研究儿童NSE及IGF-1在血清中的表达差异。结果 结果显示，对照组儿童IGF-1、NSE分别为（14.48±2.13）μmol/L、（7.11±1.19）μg/L，观察组患者对应数据为（8.82±2.96）μmol/L、（20.35±8.91）μg/L，可见，对照组IGF-1、NSE表达与观察组比较差异有统计学意义，经Pearson相关分析发现，癫痫发作期患儿血清中NSE及IGF-1二者的表达变化间呈明显的正相关（r=0.86，P<0.05）；观察组平行联合检测阳性为16例，系列联合检测阳性为0例，尽管平行联合检测灵敏度高于系列聯合检测，但系列联合检测的准确度、特异性均明显高于平行联合检测，差异有统计学意义（P<0.05）。结论 对于癫痫患儿的诊断，检测其体内NSE及IGF-1二者联合浓度表达情况具有高敏感性与特异性，值得推广应用。

[关键词] 血清；NSE；癫痫发作；儿童；IGF-1；ELISA；放射免疫法

[中图分类号] R446 [文献标识码] A [文章编号] 1674-0742（2018）01（a）-0037-03

Expression and Clinical Significance of NSE and IGF-1 in Serum of Epileptic Children

NI Guang

Department of Inner Medicine， Section Two， Qingshui Country Hospital，Qingshui， Gansu Province，741400 China

[Abstract] Objective This paper tries to further explore the NSE and IGF-1 in serum of children with clinical expression and its clinical significance in epilepsy research. Methods 120 cases of pediatric epilepsy patients were retrospectively analyzed from July 2015 to July 2017 as the observation group， another 120 cases of healthy children were convenient selected as control group. The two groups of children were tested by ELISA and radioimmunoassay， compared two groups of childrens NSE and IGF-1 in serum expression differences. Results The results showed that the patients in the control group IGF-1， NSE was （14.48±2.13）μmol/L， （7.11±1.19）μg/L， the corresponding data of patients in the observation group were （8.82±2.96）μmol/L， （20.35±8.91）μg/L， the control group IGF-1， the expression of NSE and the observation group had significant difference， the Pearson correlation analysis found that seizures were significantly and positively related to the expression changes of NSE and IGF-1 in serum of children with stage two the room （r=0.86， P<0.05）； observation group parallel combined test positive was 16 cases， a series of joint detection positive rate was 0 cases， while parallel coupling combined detection sensitivity was higher than the series of joint detection， a series of combined detection accuracy and specificity were significantly higher than that of parallel joint detection， with statistically significant difference（P<0.05）. Conclusion For the diagnosis of children with epilepsy， the NSE and IGF-1 combined with the expression of high concentration sensibility and specificity， it is worthy of popularization and application.

[Key words] Serum； NSE； Epilepsy； Children； IGF-1； ELISA； Radioimmunoassay

癫痫为儿童常见的神经病变，其发病形式多变，并且其发病时间也无法预测，因此临床医师很难观察到患者发病时的状况，特别是婴幼儿在发病时不能叙述其感受，这使小儿癫痫的诊断和鉴别诊断更有难度了。脑电图检查时小儿常常不主动配合并且很难准确及时地捕捉到异常放电的现象。而传统的检查方法主要是通过检测脑脊液来检查辅助诊断癫痫病变，这种检查方法对患儿的创伤比较大，因此近年来的相关研究结果中显示可通过检测静脉血液中相关物质的浓度间接反映脑损伤的程度。这是目前有助诊断癫痫的一个新的发展方向[1]。该次研究方便选择2015年7月—2017年7月于该院儿科接受检查治疗的120例癫痫发作患儿为研究对象，就小儿血清中的胰岛素生长因子-1（IGF-1）和神经元特异性烯醇化酶（NSE）对癫痫的发病的诊断价值进行探讨和研究，现报道如下。……