唐卿雁 陈庚 卢迎春 张广辉 赵艳 张宪民 杨生超

摘 要 建立同时测定三七地上和地下部分人参皂苷Rb1、Rb3、Rc和三七皂苷Fc含量的高效液相色谱方法。采用Agilent Zorbax EC-C18（4.6 mm×100 mm，2.7 μm）色谱柱，流动相为乙腈-0.2%磷酸水溶液，梯度洗脱，流速1 mL/min，检测波长203 nm，柱温40℃。结果表明：人参皂苷Rb1、Rb3、Rc和三七皂苷Fc分別在0.062～18.479、0.060～17.870、0.063～18.556、0.064～19.169 μg，呈良好线性关系（r>0.999 95）；三七中4种皂苷的平均回收率（n=6）分别为99.53%、100.56%、99.90%、99.60%，RSD分别为0.54%、0.60%、0.49%、0.55%。在三七地上部分和地下部分各组织中，人参皂苷Rb1在芦头部位的含量最高，人参皂苷Rb3、人参皂苷Rc和三七皂苷Fc的含量均在叶片中最高。该方法简便、准确，分离效果好，适用于三七人参皂苷Rb1、Rb3、Rc和三七皂苷Fc的测定。

关键词 三七 ；高效液相色谱法 ；皂苷含量

中图分类号 R917 文献标识码 A Doi：10.12008/j.issn.1009-2196.2018.09.018

Abstract The high performance liquid chromatography （HPLC） method was established for simultaneous determination of ginsenosides Rb1， Rb3 and Rc and notoginsenoside Fc in different parts of Panax notoginseng. The Agilent Zorbax EC-C18 （4.6 mm×100 mm， 2.7 μm） column was used in the HPLC， the mobile phases were acetonitrile-0.2% phosphoric acid with gradient elution， the detection wavelength was 203 nm， the flow rate was 1.0mL/min， and the column temperature was 40℃. The results showed that ginsenosides Rb1， Rb3 and Rc and notoginsenoside Fc had good linearity relationship （r>0.999 95） in the range of 0.061 6～18.479， 0.060～17.870， 0.063～18.556 and 0.064～19.169 μg， respectively. The ginsenosides Rb1， Rb3 and Rc， and notoginsenoside Fc had average recoveries （n=6） of 99. 53%， 100.56%， 99.90 % and 99.60%， and their relative standard deviations were 0.54%， 0.60%， 0.49% and 0.55%， respectively. The results showed that the saponins content in the underground parts and aerial parts were different apparently. The content of ginsenoside Rb1 was the highest in the rhizome， while the contents of ginsenosides Rb3 and Rc， and notoginsenoside Fc were the highest in the leaves. This HPLC method was simple and accurate with better separation effect， and hence was suitable for determination of ginsenosides Rb1， Rb3， Rc and notoginsenoside Fc.

Keywords Panax notoginseng ； high performance liquid chromatography ； saponins content

三七[Panax notoginseng （Burk.）F. H. Chen]是五加科人参属多年生草本植物，又名山漆、金不换、血参、田山七等[1]。有近600年的使用历史和400年的栽培历史[2]，其最适栽培区主要分布在我国云南、广西等地[3]。三七具有散瘀止血、消肿定痛、防止脑缺血、降脂和保护肝脏等功效[4-5]，与人参共享“北参南七”的美称。人们的传统习惯多以三七的根及根茎入药， 现已开发以三七为主要原料的药品、保健品、化妆品达数百种，如云南白药、复方丹参滴丸、血塞通、片仔癀等[6]。人参皂苷是三七的主要活性物质，目前已从三七的不同部位分离得到70余种单体皂苷[7-8]。人参皂苷按照其苷元结构可划分为3类，即达玛烷型、齐墩果烷型和奥克梯隆型。达玛烷型人参皂苷按照苷元上连接的羟基不同又可分为原人参二醇型和原人参三醇型[9]。三……