张琰 戴彰 朱建辉

[摘要] 目的 分析乙肝病毒血清学检测采用化学发光法与酶联免疫方法检验效果。方法 方便选取2017年2月—2018年5月该院收治的500例疑似乙肝患者作为观察对象，对患者采血之后血清标本采用化学发光法与酶联免疫吸附法进行检测，观察两种检测方式的检验效果。 结果 化学发光法检出乙肝病毒阳性的患者有320例，占比64.0%，酶联免疫吸附试验法检出乙肝病毒阳性患者有200例，占比40.0%，酶联免疫吸附法检出乙肝病毒阳性率要明显低于化学发光检查方法，两组数据差异有统计意义（χ2=57.69，P<0.05）。两种检查方式的重复性结果比较，化学发光检测法重复性高60例（12.0%）、中70例（14.0%）、低100例（20.0%），显著优于酶联吸附实验法，且差异有统计学意义（χ2=24.39、22.50、18.71，P<0.05）。 结论 化学发光法和酶联免疫吸附试验法检验方式相比，化学发光法对乙肝病毒阳性检出率要明显高于酶联免疫吸附试验法，对其进行定性定量检测，能够提高检测准确度。

[关键词] 乙肝病毒；血清学检测；化学发光法；酶联免疫吸附试验法；临床价值

[中图分类号] R512.6；R446.6 [文献标识码] A [文章编号] 1674-0742（2018）09（b）-0193-03

[Abstract] Objective To analyze the serological detection of hepatitis B virus by chemiluminescence method and enzyme-linked immunosorbent assay. Methods A total of 500 suspected hepatitis B patients admitted to the hospital from February 2017 to May 2018 were selected as subjects. The blood samples were convenient collected by chemiluminescence and enzyme-linked immunosorbent assay after blood collection. Results There were 320 cases of hepatitis B virus positive by chemiluminescence method， accounting for 64.0%. 200 cases of hepatitis B virus positive patients were detected by enzyme-linked immunosorbent assay （ELISA）， accounting for 40.0%， and HBV was detected by enzyme-linked immunosorbent assay. The positive rate was significantly lower than that of chemiluminescence. The difference between the two groups was statistically significant （χ2=57.69， P<0.05）. The reproducibility results of the two examination methods were 60 case（12.0%）， 70 case（14.0%）， and 100 case（20.0%）， which were significantly better than the enzyme-linked adsorption method. The difference was statistically significant （χ2=24.39， 22.50， 18.71， P<0.05）. Conclusion Compared with the enzyme-linked immunosorbent assay （ELISA）， the positive detection rate of hepatitis B virus by chemiluminescence method is significantly higher than that of enzyme-linked immunosorbent assay （ELISA） qualitative and quantitative detection can improve the detection accuracy.

[Key words] Hepatitis B virus； Serological detection； Chemiluminescence method； Enzyme-linked immunosorbent assay； Clinical value

乙型肝炎病毒在我國的发病率相对较高，目前没有针对乙型肝炎疾病特效治疗方式，针对乙型肝炎病毒实施早期诊断治疗至关重要。对乙型肝炎病毒检测的手段主要是化学发光法和酶联免疫吸附试验法两种检测方式，这两种检测方式在临床上均具有重要应用价值[1]。现就2017年2月—2018年5月该院收治的500例疑似乙肝患者作为观察对象，分析乙肝病毒血清学检测采用化学发光法与酶联免疫方法检验效果，现报道如下。

1 资料与方法

1.1 一般资料

方便选取该院收治的500例疑似乙肝患者作为观察对象，500例患者中男性患者有260例，女性患者有240例，年龄均在20～60岁之间，平均年龄为（42.8±5.8）岁。500例患者诊断标准均根据《乙型病毒性肝炎诊断标准》进行检查诊断，且所选病例均通过伦理委员会的批准，患者及家属均知情同意。……