崔佳雯+++马书杰++褚立希

[摘要] 目的 探討针刺百会穴结合游泳训练对大脑中动脉缺血再灌注大鼠认知功能的影响。 方法 将SD大鼠手术制成右侧大脑中动脉缺血再灌注模型，将手术模型成功后的28只大鼠进行神经功能评定及水迷宫测试后按照随机数字表分为模型组、针刺组、游泳组和针游结合组，每组7只；另设假手术组7只。假手术组及模型组不给予任何治疗；针刺组给予百会穴针刺治疗，1次/d；游泳组给予游泳训练，1次/d；针游结合组给予针刺百会穴结合游泳训练，1次/d。共治疗6 d。每天治疗前测量体重进行神经功能评分，治疗6 d后进行水迷宫测试其学习记忆能力，测试结束取脑，采用TUNEL染色观察海马区细胞凋亡情况。 结果 治疗后针刺和游泳治疗无明显交互作用（P > 0.05），但针游结合组大鼠神经功能评分低于模型组（P < 0.05）；针游结合组大鼠水迷宫穿越平台次数多于模型组（P < 0.05）。 结论 针刺结合游泳训练对于大脑中动脉缺血再灌注大鼠认知功能的影响可能优于单纯针刺或游泳，需要更深入的研究探索。

[关键词] 大脑中动脉缺血再灌注；大鼠；针刺；游泳训练；Morris水迷宫；海马区；细胞凋亡

[中图分类号] R743.5 [文献标识码] A [文章编号] 1673-7210（2017）09（b）-0025-04

The influence of acupuncture combined with swimming training for the cognitive function in middle cerebral artery ischemia-reperfusion rats

CUI Jiawen MA Shujie CHU Lixi

College of Rehabilitation Medicine， Shanghai University of Traditional Chinese Medicine， Shanghai 201203， China

[Abstract] Objective To observe the influence of acupuncture combined with swimming training for the cognitive function in middle cerebral artery ischemia-reperfusion rats. Methods SD rats were made into the model of right middle cerebral artery ischemia-reperfusion. After evaluation of neurological function test and water maze test operation， the 28 successful model rats were divided into model group， acupuncture group， swimming training group， and integrated acupuncture and swimming training group by random number table， with 7 rats in each group， 7 rats of sham-operation group were set separately. There was no treatment for sham-operation group and model group， the acupuncture group was given acupuncture therapy on GV 20， once per day， while the swimming training group was given swimming training， once per day， and integrated acupuncture and swimming training group was given integrated acupuncture therapy on GV 20 and swimming training， once per day. Total treatment time was 6 days. Each day before treatment， weight and neural functional test were evaluated. After treatment for 6 days， water maze test was detected for the ability of learning and remembering， after all treatment and evaluation， the brains were taken， then TUNEL staining was used to observe the cell apoptosis in hippocampus. Results After treatment， there was no interactive effect between acupuncture and swimming training （P > 0.05）， the neural function score of integrated acupuncture and swimming training group was lower than that of model group （P < 0.05）， the number of step through in water maze test of integrated acupuncture and swimming training group was more than that of model group （P < 0.05）. Conclusion The influence of acupuncture and swimming training for the cognitive function in middle cerebral artery ischemia-reperfusion rats may be better than acupuncture or swimming training only， more research needs to be done in the future.endprint

[Key words] Middle cerebral artery ischemia-reperfusion； Rats； Acupuncture； Swimming training； Morris water maze； Hippocampus； Cell apoptosis

研究表明，脑卒中后认知功能障碍的发生率可达72.5%[1]。脑卒中患者的认知功能可以提高患者的理解力和执行力，从而提高康复效益[2]。因此脑卒中后的认知功能损害越来越受到重视，同时也已成为神经科学领域当前研究发展的重点[3]。Quaney等[4]研究表明有氧运动能提高脑卒中患者的信息处理速度、运动学习能力、内隐记忆和运动功能。针灸可通过改善血液流变性障碍、调节中枢胆碱能递质、调节神经肽、调节血管活性物质功能、清除自由基、阻止细胞凋亡、保护神经元等途径改善患者认知功能[5-7]。针刺……