王昱

(陇南师范高等专科学校农林技术学院，陇南特色农业生物资源研究开发中心，甘肃成县742500)

油橄榄叶提取物对大鼠酒精性脂肪肝的保护作用

王昱

(陇南师范高等专科学校农林技术学院，陇南特色农业生物资源研究开发中心，甘肃成县742500)

目的观察油橄榄叶提取物(OLE)对酒精性肝损伤的保护作用及机制。方法用递增法灌胃乙醇24周建立大鼠肝损伤模型，造模同时用OLE(250 mg·kg-1、500 mg·kg-1、1 000 mg·kg-1)进行灌胃治疗。利用生物显微技术观察肝脏组织结构的变化，检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、甘油三酯(TG)和总胆固醇(TC)水平，用放射免疫法检测肝脏肿瘤坏死因子α(TNF-α)、白细胞介素1(IL-1β)的含量，用比色法检测肝脏超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)活性及丙二醛(MDA)含量，用免疫组织化学法检测固醇调节元件结合蛋白-1c(SREBP-1c)的表达情况。结果与模型组比较，OLE治疗后，血清ALT、AST、TG、TC水平降低；肝脏TNF-α、IL-1β、MDA含量及SREBP-1c表达呈降低趋势；SOD、CAT、GR水平均显著升高；肝脏损伤程度明显减轻。结论OLE对酒精性肝损伤有一定的保护作用，可能与其减少自由基损伤、减轻炎症反应、抑制SREBP-1c表达有关。

油橄榄叶提取物； 酒精； 炎症因子； 固醇调节元件结合蛋白-1c； 抗氧化能力

Abstract：ObjectiveExplore the protective role of olive leaf extract (OLE) on alcohol hepatic injury.MethodsA hepatic injury model of rat was established by using edible alcohol with increasing dosages for 24 weeks. And then the rats were given OLE at the doses of 250 mg·kg-1， 500 mg·kg-1or 1 000 mg·kg-1by intragastric administration. The changes of liver histological structure were observed by bio-microscopy. The levels of alanine aminotransferase (ALT)， aspartate aminotransferase (AST)， triglyceride (TG)， and total cholesterol (TC) in serum were tested. The contents of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were detected by radioimmunoassay and the activities of superoxide dismutase (SOD)， catalase (CAT)， glutathione reductase (GR) and malondialdehyde (MDA) content in liver were determined by spectrophotometry， and the expression of sterol regulatory element-binding protein 1c (SREBP-1c) in liver were analyzed by immunohistochemistry.ResultsCompared with the model group， the treatments of OLE could significantly decrease the levels of TG， TC， ALT， AST， TNF-α， IL-1β， MDA and SREBP-1c expression， while the activities of SOD， CAT and GR were significantly increased. Furthermore， the liver injury was alleviated along with the treatment of OLE.ConclusionOLE has a protective effect on alcohol hepatic injury， and this may be due to the reduced free radical lesion， relieved inflammatory response and inhibited SREBP-1c expression.

Keywords： olive leaf extract； alcohol； inflammatory factors； SREBP-1c； antioxidant capacity

脂肪变性是酒精性肝病最早和最常出现的损伤形式，长期酗酒者约90%以上可发展成脂肪肝，若不加重视，可在短期内发展为不可逆的肝损伤。研究表明，发生酒精性脂肪肝后，肝细胞肿大压迫肝血窦造成窦间隙变窄，使肝细胞缺氧，长时间就会导致肝细胞变性和坏死，并促进肝纤维化的发生(祁瑞瑞，2012)。……