王伟+文欢+汪颀浩+张大燕+彭成+高继海

摘要：以广元市旺苍县4个样区136份杜仲树皮为试材，采用HPLC法测定其绿原酸和松脂醇二葡萄糖苷含量，活立木树皮产量测算方法测算树皮产量，研究杜仲树皮产量及活性成分含量与树龄、株高、胸径及皮厚的相关性，为旺苍县杜仲优良品种的选育提供有效参考。结果表明：4个样区的杜仲树皮绿原酸含量0.079%～1.208%，松脂醇二葡萄糖苷含量0.068%～5.251%，树皮平均年产量0.03～2.56 kg。树龄为6～28年杜仲树皮，活性成分含量主要与树龄、胸径呈负相关，产量与树龄、株高、胸径、皮厚呈正相关。因此，可以将树龄和胸径2个指标优先纳入旺苍县优良杜仲的选育工作中。

关键词：杜仲树皮；活性成分；产量；生长指标；相关性分析

中图分类号：S567.1+90.1 文献标识号：A 文章编号：1001-4942（2017）08-0055-05

Abstract Using 136 bark samples from 4 areas of Wangcang County， Guangyuan City， the correlations of bark yield and active ingredient contents with age， plant height， diameter at breast height and bark thickness of Eucommia ulmoides were studied to provide references for breeding good varieties. The content of active ingredients in E. ulmoides barks was determined by HPLC method， and the bark yield was calculated in the alive condition. The results showed that the content of chlorogenic acid and pinoresinol diglucoside in E. ulmoides barks was 0.079%～1.208% and 0.068%～5.251% respectively， and the average annual bark yield was 0.03～2.56 kg. In the 6～28-year-old samples， the content of active ingredients was mainly negtively correlated with plant age and diameter at breast height， and the bark yield was positively correlated with the age， plant height， diameter at breast height and bark thickness. Therefore， tree age and diameter at breast height could be chosen as the prior indexes for variety breeding.

Keywords Eucommia ulmoides bark； Active ingredient； Yield； Growth and development characters； Correlation analysis

杜仲（Eucommia ulmoides Oliv.）為杜仲科杜仲属植物，分布于我国秦岭、黄河以南、五岭以北、黄海以西及云南高原以东区域，其间基本上是长江中下游流域[1]。其树皮别名丝绵皮、玉丝皮，是我国特色的名贵中药材[2]。杜仲在《神农本草经》中被作为上品药物，具有降血压、抗氧化、抗疲劳、增强免疫力、抗骨质疏松症、抗肿瘤、护肝、降血糖、抗衰老等功效[3]。

杜仲在我国的应用历史长达两千多年[4]，但其资源已遭到严重破坏，杜仲大树几乎被毁殆尽[5]，目前已被国家列入重点保护对象。国外于19世纪末开始引进杜仲，20世纪对其应用研究开始快速发展[6]。我国学者于20世纪80年代初，针对杜仲产量、抗逆性等方面，已对杜仲资源的选育工作进行了研究[7]。……