孙瑜嬬 孙理军 孙耀光 张琪 刘熙如 柏云飞

摘要：目的 探討补肾中药对肾虚体质大鼠生长发育及脑组织胆碱乙酰化酶（CHAc）含量的调节作用。方法 采用“猫吓鼠”方法制作先天不足加后天失养复合型肾虚模型。造模大鼠随机分为模型组、左归丸组和右归丸组，空白组大鼠产自正常孕鼠。对仔鼠恐吓同时开始灌胃给药，药物组给予左归丸和右归丸混悬液，空白组和模型组予等量生理盐水，每日1次，连续3个月。观察大鼠生长发育一般状况，记录5～8周龄各组大鼠体质量和平均食物利用率，ELISA检测大鼠脑组织CHAc含量。结果 仔鼠5～8周龄时，模型组较空白组体质量明显减少（P<0.01），左归丸组和右归丸组体质量较模型组明显增加（P<0.05，P<0.01），平均食物利用率与体质量呈正相关；模型组脑组织CHAc含量较空白组明显降低（P<0.01），左归丸组、右归丸组较模型组明显升高（P<0.01）。结论 补肾中药可改善肾虚质大鼠生长发育落后状态并调节脑内CHAc含量，从而提高肾虚质大鼠学习记忆能力。

关键词：补肾中药；生长发育；胆碱乙酰化酶；大鼠

DOI：10.3969/j.issn.1005-5304.2017.09.012

中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2017）09-0048-03

Effects of Kidney Reinforcing Medicine on Growth and Development and Brain Choline Acetyl Transferas Content of Rats with Kidney-deficiency Constitution SUN Yu-ru， SUN Li-jun， SUN Yao-guang， ZHANG Qi， LIU Xi-ru， BO Yun-fei （Shaanxi University of Chinese Medicine， Xianyang 712046， China）

Abstract： Objective To discuss the adjustment effects of kidney reinforcing medicine on growth and development and the content of choline acetyl transferase （CHAc） in rats with kidney-deficiency constitution. Methods The method of “cats scare rats” was used to build composite offspring rat models with deficiency and acquired dystrophy， and then the models were divided into model group， Zuogui Pill group and Yougui Pill group. The rats in blank group came from normal pregnant rats. Baby rats were scared and received gavage at the same time. All administration groups received suspension of Zuigui Pills or Yougui Pills. Blank group and model group received the same amount of normal saline， once a day， for 3 months. Growth and development of rats were observed. Weight and food utilization of rats aged 5–8 weeks in each group were recorded. The content of brain CHAc was detected by ELISA. Results When offspring were 5–8 weeks old， weights of rats in the model group were lower than blank group （P<0.01）； while weights of rats in Zuogui Pill group and Yougui Pill group increased significantly （P<0.05） and the food utilization was positively correlated with weight； the CHAc content in the model group decreased significantly compared with blank group （P<0.05， P<0.01）； while the CHAc content in Zuogui Pill group and Yougui Pill group increased significantly compared with the model group （P<0.01）. Conclusion Kidney reinforcing medicine can improve backward growth and development of rats with kidney-deficiency constitution and adjust CHAc in the brain， so as to promote the learning and memory ability of rats with kidney-deficiency constitution.

Key words： kidney reinforcing medicine； growth and development； CHAc； rats

基金项目：陕西省重点实验室研究项目（15JS026）

通讯作者：孙理军，E-mail：1246503262@qq.com

以肾中精气亏虚、肾脏功能低下为主要表现的肾虚体质，是各类人群中常见的体质类型之一。中医理论有“肾主生殖、主蛰守位、在志为恐、在窍二阴”。前期研究表明，肾虚体质模型动物较正常群体出现精神萎靡、瑟缩不动、敏感易惊、饮食饮水量少、体质量偏轻、生长发育迟缓、皮毛松弛、毛发稀疏等一系列临床现象[1]。一方面“肾藏精生髓”，肾精不足无以充髓实脑而致脑失其用；另一方面“恐伤肾”，恐惧不解则致肾精亏虚、脑窍失养。本研究以肾虚体质模型大鼠生长发育情况及脑组织胆碱乙酰化酶（CHAc）含量为研究对象，探讨补肾中药对肾虚体质生长发育及脑功能衰退的调节作用。……