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麝香草酚对13种植物病原微生物的抑制作用

2017-07-13程翠利韩康卢向阳方俊田云蒋红梅

安徽农业科学 2017年24期

程翠利 韩康 卢向阳 方俊 田云 蒋红梅

摘要 [目的]探讨石香薷主要成分麝香草酚对植物病原菌的影响。[方法]采用管碟法研究麝香草酚对13种致病微生物的抑制作用。[结果]麝香草酚对13种受试菌均有不同程度的抑制作用,其中对水稻恶苗病菌、黄瓜枯萎病菌、黄瓜炭疽病菌、油菜菌核病菌4种植物病害真菌的抑制效果极显著,抑菌效果与麝香草酚浓度呈正相关,对这4种真菌的最低抑菌浓度(MIC)分别为0.125、0.063、0.063、0.250 mg/mL,最低杀菌浓度(MBC)分别为0.125、0.125、0.063、3.000 mg/mL。[结论]试验结果可为麝香草酚的进一步研究提供理论依据。

关键词 麝香草酚;病原微生物;最低抑菌浓度(MIC);最低杀菌浓度(MBC)

Inhibitory Activity of Thymol on 13 Kinds of Plant Pathogenic Microorganism

CHENG Cui-li1,HAN Kang1,LU Xiang-yang2,JIANG Hong-mei1* et al (1.College of Science,Hunan Agricultural University,Changsha,Hunan 410128;2.College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha,Hunan 410128)

Abstract [Objective] The aim was to explore effects of thymol on the plant pathogenic microorganism.[Method] We studied the inhibitory activity of thymol on 13 kinds of pathogenic microorganism by cup-plate method.[Result] The thymol could effectively inhibit the growth of tested kinds of bacteria,and could inhibit significantly four kinds of plant disease fungus,which were Fusarium moniliforme Sheldon,Fusarium oxysporum Schlecht,Colletorichum orbiculare and Sclerotinia sclerotiorum.The bacteriostatic effect was positive correlation to the concentration of thymol.The minimum inhibitory concentration(MIC) of thymol on four kinds of plant disease fungus were 0.125,0.063,0.063 and 0.250 mg/mL,and the minimum bactericidal concentration (MBC) were 0.125,0.125,0.063 and 3.000 mg/mL,respectively.[Conclusion] The results provide reference for further research on thymol.

Key words Thymol;Pathogenic microorganisms;MIC;MBC

石香薷(Mosla chinensis Maxim)别名细叶香薷,是唇型科的一年生草本植物[1],在长江以南地域有着非常广泛的分布,是我国药典收载的正品香薷之一,《本草纲目》《名医别录》《植物名实图考》《本草品汇精要》均有记载。全草入药,性温,味辛凉,是发汗解表、和中利湿的去暑良药。该草药主治中暑发烧、伤风恶寒、急性肠胃炎、跌打肿痛、牙龈肿痛、下肢水肿、颜面浮肿、皮肤湿疹、瘙痒、多发性疥肿、毒蛇咬伤等[2-3]。石香薷主要成分百里香酚与麝香草酚对食品污染微生物、人畜感染菌表现出广谱的抗菌能力,但麝香草酚对植物病原菌的影响研究较少。鉴于此,笔者研究了石香薷主要成分麝香草酚对13种植物病原微生物的抑菌作用,旨在为麝香草酚的进一步研究提供参考。

1 材料与方法

1.1 材料

1.1.1 供试菌。

真菌类:水稻恶苗病菌(Fusarium moniliforme)、棉花枯萎病菌(Fusarium oxysporum)、黄瓜枯……

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