刘雅辉 左永梅 孙建平

摘要 [目的]研究咸水胁迫对菘蓝种子萌发的影响及其耐盐阈值。[方法]利用培养皿滤纸发芽试验，分析不同浓度地下咸水对菘蓝种子萌发的影响，通过相关性和回归分析，确定菘蓝萌发期的耐盐鉴定指标和耐盐阈值。[结果]咸水浓度低于0.4%时，菘蓝种子萌发基本不受影响，当咸水浓度高于0.6%时，菘蓝种子萌发的所有指标的相对值均受到明显抑制；相对发芽率与咸水浓度间呈线性关系且高度相关，大叶菘蓝和小叶菘蓝的耐盐阈值分别为0.66%和0.73%，可见菘蓝种子萌发期的耐盐性较强，小叶菘蓝的耐受性更高，适于在中、重滨海盐碱地种植。[结论]该研究为菘蓝资源的耐盐性鉴定及利用提供了理论依据。

关键词 咸水胁迫；菘蓝；种子萌发；耐盐阈值

Effect of Salt Water Stress on Seed Germination of Isatis indigotica and Analysis of Salt Tolerance Threshold

LIU Ya-hui，ZUO Yong-mei，SUN Jian-ping （Institute of Coastal Agricultural，Hebei Academy of Agriculture and Forestry Sciences，Saline and Alkali Land Greening Engineering Technology Center of Heibei Province，Tangshan Key Laboratory of Plant Salt Tolerance Research，Tangshan，Hebei 063299）

Abstract [Objective]To study the effect of salt water stress on seed germination of Isatis indigotica and analyze the salt tolerance threshold.[Method]Filter paper germination test was carried out to study the effects of coastal saline alkali soil on germination of I.indigotica，salt tolerance index and salt tolerance threshold in the seedling stage of I.indigotica were determined through correlation analysis and regression analysis.[Result]Germination of I.indigotica was not affected when concentration of salt water was lower than 0.4%.The relative value of all indexes of germination were significantly inhibited when concentration of salt water was higher than 0.6%.The relative germination rate was linearly related to salt water concentration and highly correlated.The salt tolerance thresholds of daye and lobular I.indigotica were 0.66%，0.73% respectively，it was evident that I.indigotica had strong salt tolerance in germination，which salt tolerance of lobular I.indigotica was higher than daye I.indigotica，it was suitable for planting in light and moderate saline alkali area.[Conclusion]The study provided theoretical basis for the selection and utilization of salt tolerant I.indigotica.

Key words Salt water stress；Isatis indigotica；Seed germination；Salt tolerance threshold

土壤盐渍化一直是限制农业生产的世界性问题[1-3]。滨海盐渍土是盐渍化土壤的一种类型，土壤NaCl含量高，通透性差，严重影响作物的生长。不同植物或同一种植物不同品种间的耐盐性不同，通过挖掘植物本身的耐盐能力，筛选和培育耐盐植物品种是开发利用盐渍土的有效途径之一[4]。研究发现[5]，一些中草药植物具有较强的耐盐性，而且在盐碱地种植可以提高药用成分，因此筛选耐盐中草药植物，发展盐碱地中药种植产业，不仅能够高效利用盐土资源，改良修复盐碱地，还可为滨海盐碱区农业种植结构调整、农民增收、农业增效探索新途径，为滨海现代农业发展提供新模式。……