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摘要：目的优选柴黄清胰活血颗粒的最佳乙醇提取工艺。方法采用乙醇回流提取法、乙醇超声提取法和乙醇渗漉提取法，提取柴黄清胰活血颗粒中部分中药材，用HPLC法测定其指标性成分延胡索乙素的含量，选取延胡索乙素含量最高的提取方法作为柴黄清胰活血颗粒的乙醇提取工艺，并通过正交设计法优选其最佳提取工艺。结果乙醇回流提取法与乙醇渗漉法提取的延胡索乙素含量较高，且含量相差不大，结合实践，采用乙醇渗漉法提取延胡索乙素，其最佳渗漉提取工艺为：浸泡36 h，乙醇浓度55%，乙醇用量10倍，渗漉流速3 ml·min-1。结论该工艺客观可行，稳定合理，可为工艺生产提供理论依据。

关键词：渗漉提取法；正交设计；高效液相色谱；延胡索乙素；柴黄清胰活血颗粒

中图分类号：R284文献标志码：A文章编号：1007-2349（2017）06-0074-03

【Abstract】Objective： To optimize the best ethanol extraction process of Chaihuang Qingyi Huoxue Granule. Methods： Ethanol reflux extraction， ethanol ultrasonic extraction and ethanol percolation extraction were used to extract part of medicinal materials from Chaihuang Qingyi Huoxue Granule and HPLC was used to determine content of tetrahydropalmatine. The highest content extraction of tetrahydropalmatine was taken as ethanol extraction technology of Chaihuang Qingyi Huoxue Granule， and its optimal extraction process was optimized by orthogonal design. Results： The ethanol reflux extraction and ethanol percolation extraction could extract higher amount of content with less different content. In practice， the ethanol percolation extraction was used to extract tetrahydropalmatine. The optimum extraction process was as follows： 36-hour soaking， 55% of ethanol concentration， 10 times of ethanol dosage， 3 ml·min-1 of percolation flow rate. Conclusion： The process is objective and feasible， stable and reasonable， which can provide a theoretical basis for its production.

【Key words】percolation extraction， orthogonal design， HPLC， tetrahydropalmatine， Chaihuang Qingyi Huoxue Granule

柴黄清胰活血颗粒是西南医科大学附属中医医院协定处方，主要由生大黄、柴胡、延胡索、丹参、栀子、黄芩、白芍等14味中药组成。具有清热解毒、活血消肿、通腑泻热、理气止痛的功效，用于急性胰腺炎的治疗。处方中延胡索所含有效成分延胡索乙素和丹参所含有效成分：丹参酮ⅡA、丹参酮ⅡB、隐丹参酮、异隐丹参酮等成分，易溶于醇、醚等有机溶剂[1～2]。现代药理研究证实，延胡索乙素具有镇痛抗炎作用，提高模型大鼠应激能力，提高小鼠抗疲劳能力及耐缺氧能力等作用[3]。为了最大限度地提取上述中药的有效成分，笔者考虑使……