蔡红蝶++宿树兰+郭盛+朱悦+钱大玮+陶伟伟+段金廒

[摘要] 黃蜀葵花中富含黄酮类成分，具有保护心血管、改善肾功能等作用。该文采用3T3-L1前脂肪细胞诱导分化的成熟脂肪细胞，建立胰岛素抵抗模型，分为正常组，模型组，异槲皮苷（HY）、金丝桃苷（JY）、槲皮素（QT）、槲皮素-3′-O-葡萄糖苷（QG）、棉皮素-8-O-β-葡萄糖醛酸（GG）给药组，BCA法检测细胞培养液中葡萄糖的含量，荧光定量qPCR检测PPARγ，C/EBPα，SREBP-1，脂联素，内酯素，抵抗素基因mRNA的表达水平。结果显示，5种黄酮类化合物5 μmol·L^-1浓度时可促进3T3-L1前脂肪细胞的增殖，浓度为100 μmol·L^-1时可抑制前脂肪细胞的增殖；与正常组比较，模型组细胞对葡萄糖摄取量降低（P<0.01）；与模型组相比，5种黄酮类化合物100 μmol·L^-1给药组细胞葡萄糖消耗量显著上升（P<0.01），PPARγ，C/EBPα，脂联素表达明显增加（P<0.01），SREBP-1，抵抗素，内酯素的表达明显降低（P<0.01），促使脂肪细胞分化。研究表明，HY，JY，QT，QG，GG能调控前脂肪细胞增殖，促进脂肪细胞分化及糖脂代谢相关因子PPARγ，C/EBPα，SREBP-1，脂联素，内酯素，抵抗素的表达，促进前脂肪细胞分化，增加葡萄糖利用，从而改善胰岛素抵抗。

[关键词] 锦葵科；黄属葵花；胰岛素抵抗；黄酮类成分；3T3-L1脂肪细胞

[Abstract] Abelmoschus manihot was rich in flavonoids，which has been reported the activity on protecting angiocarpy and improving renal function. This study aimed to explore the action mechanism of five flavonoids from A. manihot on how to ameliorating insulin resistance through the regulation of the glucose and expression of PPARγ，C/EBPα，SREBP-1，resistin，visfatin，adiponectin in 3T3-L1 adipocytes. After the 3T3-L1 preadipocytes were differentiated into mature adipocytes，insulin resistance model was built. Insulin resistance adipocytes were treated with 5，100 μmol·L^-1 quercetin，isoquercitrin，hyperoside，quercitrin-3′-O-glucoside，gossypetin-8-O-β-glucoside. The glucose was indirectly determined by BCA kit. The mRNA expression levels of PPARγ，C/EBPα，SREBP-1，resistin，visfatin，adiponectin were detected by real-time quantitative PCR. Results showed that five flavonoids at 5 μmol·L^-1 could accelerate preadipocytes proliferation and inhibit that at 100 μmol·L^-1 Compared with the normal group，glucose uptake reduced significantly in model group （P<0.01）. With the treatment of five flavonoids at 100 μmol·L^-1，glucose consumption increased significantly （P<0.01）. The high expression of PPARγ，C/EBPα，adiponectin expression was significantly increased （P<0.01），and low expression of SREBP-1，resistin，visfatin after respective administration with five flavonoids at 100 μmol·L^-1 promoted adipocyte differentiation. This study showed that，HY，JY，QT，QG，GG can control preadipocytes proliferation，promote adipocyte differentiation and regulate the expression of relative factors with lipid metabolism，such as PPARγ，C/EBPα，SREBP-1，adiponectin，resistin，visfatin，increasing glucose utilization and improving insulin resistance in 3T3-L1 adipocyte.

[Key words] Malvaceae；Abelmoschus manihot；insulin resistance；flavonoids；3T3-L1 adipocyte

doi：10.4268/cjcmm20162424

黄蜀葵花为锦葵科Malvaceae秋葵属Abelmoschus黄蜀葵Abelmoschus manihot （L.） Medic.的干燥花冠，始载于《嘉佑本草》，先后收录于《本草纲目》与2010年版《中国药典》。其味甘、辛；性凉；归心、肾、膀胱经；具有利尿通淋、活血、止血、消肿解毒的功效，主淋证，吐血，衄血，崩漏，胎衣不下，痈肿疮毒，水火烫伤。药理研究表明，黄蜀葵花具有保护心脑缺血损伤、改善肾功能、抗炎、抗氧化、抗病毒等各种生物活性[1-5]。化学成分研究发现，黄蜀葵花中主要含有黄酮类、还原糖类、鞣酸类及长链烃类等成分，其中黄酮类成分质量分数约占6%[6]，为其主要活性物质，主要包括金丝桃苷、异槲皮苷、槲皮素、槲皮素-3′-O-葡萄糖苷[7-8]。据报道[9]，总黄酮能降低链脲佐菌素诱导的糖尿病小鼠的血糖，显著促进3T3-L1脂肪细胞对葡萄糖的吸收。……