静脉血样品放置时间对血细胞分析结果的影响
2016-10-28郭琛峰
郭琛峰
摘 要 目的:研究静脉血放置时间的长短对血细胞分析结果的影响。方法:选择2015年8月健康体检者30人的静脉血标本,用乙二胺四乙酸二钾抗凝,在30 min内上机测定红细胞、白细胞和血小板计数,并于送检后1、2、3、5 h进行血常规检测,以30 min内测定结果为对照,比较不同时间点所测结果。结果:在5 h内,红细胞和白细胞计数无显著变化,不同时间点所测结果与对照相比差异均无统计学意义(P>0.05);而血小板计数明显降低(P<0.01)。结论:红细胞和白细胞计数可在5 h内分析,而血小板计数应在30 min内分析。
关键词 静脉血 放置时间 血常规分析
中图分类号:R446.11+3 文献标志码:A 文章编号:1006-1533(2016)18-0032-02
Influence of storage time of venous blood samples on the results of blood cell analysis
GUO Chenfeng
( Changshou Community Health Service Center of Putuo District, Shanghai 200060, China)
ABSTRACT Objective: To study the effect of the length of storage time of venous blood on the results of blood cell analysis. Methods: In August 2015, the venous blood samples of 30 cases of health physical examination were collected, and EDTA-2K was used to anti-coagulate venous blood samples. Within 30 minutes red blood cell count, white blood cell count and platelet count were determined with the computer, and after 1 h, 2 h, 3 h, 5 h of submission, the routine blood test was used to determine the these blood cell parameters. The measured results at different time points were compared with 30 min determination results. Results: There were no significant changes in the red blood cell count and white blood cell count within 5 h, and there was no significant difference between the measured results at different time points compared with the control( P>0.05) . But platelet count decreased significantly(P<0.01). Conclusion: red blood cell count and white blood cell count can be analyzed within 5 hours, and the platelet count should be detected in 30 minutes.
KEY WORDS venous blood; storage time; blood routine analysis
血常规检查是临床最基础、最常用的血液检查之一[1],作为临床诊断的基本依据,其检查结果的准确性和精确性是体现检验质量的重要指标[2]。血液标本的放置时间是检验前质量控制的一个重要环节,对检测结果的准确性有一定影响[3]。目前,全自动血细胞分析仪的应用极为广泛,但有关标本放置时间对检测结果可靠性的影响尚无定论[4]。因此,本文对静脉血标本放置时间对测定结果的影响进行了研究,现报道如下。
1 材料与方法
1.1 标本来源
选取2015年8月本院健康体检者30人,其中男15人,女15人,年龄48~85岁,平均67.5岁。
1.2 方法
按参考文献[5]方法采集静脉血2 ml,置于含有乙二胺四乙酸二钾的抗凝管中,充分混匀(上下颠倒10次)后开始计时,分别于1、2、3、5 h时分别计数红细胞、白细胞和血小板,将30 min内所测数据作为自身对照。血细胞标本检测采用日本SysmexXS-500i全自动血细胞分析仪及配套原装进口试剂,检测前已做好质控,空白计数符合标准。……p>
