卢婷 林二培 张俊红等

摘要[目的]研究榧树雄球花发育过程中可溶性蛋白的动态变化，以进一步分离与花芽分化相关的特异蛋白。[方法]以榧树雄株花芽及芽基部叶片为材料，测定分析雄球花发育过程中可溶性蛋白含量以及特异蛋白组分的动态变化。[结果]在雄球花发育过程中，花枝叶片的可溶性蛋白存在2个表达高峰，分别在鳞片分化期和二核期花粉粒形成期；而花芽中的可溶性蛋白含量呈现一直下降的趋势。SDSPAGE分析结果也表明，在雄球花发育的不同阶段，在花枝叶片和花芽中均出现了特异的蛋白谱带。[结论]为了解榧树雄球花发育的生理过程和进一步研究香榧成花调控提供了依据。

关键词榧树；雄球花；可溶性蛋白

中图分类号S718.3文献标识码A文章编号0517-6611（2016）04-219-03

The Dynamic Changes of Soluble Proteins during Staminate Strobili Development in Torreya grandis

LU Ting1，2，LIN Erpei1，ZHANG Junhong1，LOU Xiongzhen1* et al （1.School of Forestry and Biotechnology，Zhejiang A&F University，Linan，Zhejiang 311300； 2.Yuyao Municipal Bureau of Agriculture and Forestry Seed Management Station，Yuyao，Zhejiang 315400）

Abstract[Objective] To study the dynamics of soluble protein in development process of Torreya grandis staminate strobili，and further isolate specific proteins related to flower bud differentiation.[Method] With male plant flower bud and bud base leaves as materials，the dynamics of soluble protein content and specific protein components during development were determined and analyzed.[Result] During the development of staminate strobili，there were two expression peaks for the soluble proteins in the leaves，which were shown at the microsporophyll differentiation stage and binuclear pollen stage，respectively； while in staminate strobili，the contents of soluble proteins presented the downward trend.Meanwhile，analysis of the SDSPAGE also indicated that several specific protein components were shown in the leaves and staminate strobili.[Conclusion] The study results can provide reference for understanding the physiological process of Torreya grandis staminate strobili，and further research of flowering regulation of Torreya grandis.

Key wordsTorreya grandis； Staminate strobili； Soluble proteins

香榧（Torreya grandis）屬红豆杉科榧属，是从榧树（T.grandis）的自然变异类型中经无性繁殖培育而成的优良品种，集食用、药用、油用、材用和观赏绿化于一身，栽培经济效益显著，其果实是我国特有的珍稀干果[1-3]。浙江省是我国香榧重要的产区，但香榧营养生长期长，“大小年”现象突出，严重限制其产业的持续发展[4]。从生理角度讲，营养生长期和“大小年”等生产相关的问题均与香榧花芽形成分化有关。因此，开展香榧花芽形成的生理及其调控研究，对于推动当前香榧产业的可持续发展具有重要的现实意义。

多年生木本植物花芽的形成受到复杂的内在因子和环境因素的调控，现有研究表明蛋白质含量的动态变化与植物花芽形成分化有关。……