黄胜先 王正文 李佳林等

摘要[目的]筛选有机蓝莓园大窠蓑蛾的有效生物防治药剂。[方法]采用室内毒力测定和田间药效试验，测定棉铃虫核型多角体病毒、苏云金杆菌、球孢白僵菌、绿僵菌4种目前常用的生物药剂对大窠蓑蛾幼虫的防治效果。[结果]室内毒力测定结果表明：棉铃虫核型多角体病毒的杀虫活性最高，LD50为2.15×106 PIB/mL，4种生物药剂对大窠蓑蛾幼虫毒力由高到低依次为棉铃虫核型多角体病毒、苏云金杆菌、球孢白僵菌、绿僵菌；田间药效试验结果表明：药后10 d各药剂的防治效果均超过50%，以棉铃虫核型多角体病毒处理防效最高，为94.17%。[结论]棉铃虫核型多角体病毒可作为防治大窠蓑蛾幼虫的首选药剂，建议在7～9月进行喷药，即在大窠蓑蛾幼虫期的2～3龄以前效果最好。

关键词大窠蓑蛾；有机蓝莓园；毒力测定；田间药效

中图分类号S436.63文献标识码A文章编号0517-6611（2016）04-163-02

The Indoor Toxicity of Clania variegate Snellen in Organic Blueberry Plantation and Field Efficacy

HUANG Shengxian， WANG Zhengwen， LI Jialin， CHEN Jinwu* et al（Qiandongnan Agricultural Science Institute， Kaili， Guizhou 556000）

Abstract[Objective] To screen out effective medicament against Clania variegate Snellen in organic blueberry plantation. [Method] Through indoor toxicity determination and field efficacy experiment， the control efficacy of 4 commonly used medicament （Helicoverpa armigera nuclear polyhedrosis virus， Bacillus thuringiensis， Beauveria bassiana and Metarrhizium anisopliae） against Clania variegate Snellen was determined. [Result] The results of laboratory tests showed that the insecticidal activity of Helicoverpa armigera nuclear polyhedrosis virus is the highest， LD50 for 2.15×106 PIB/mL； the toxicity from high to low is Helicoverpa armigera nuclear polyhedrosis virus>Bacillus thuringiensis>Beauveria bassiana>Metarrhizium anisopliae. The results of field efficacy showed that efficacy of each medicament after applying 10 d is above 50%， Helicoverpa armigera nuclear polyhedrosis virus is the highest as 94.17%. [Conclusion] Helicoverpa armigera nuclear polyhedrosis virus can be used as preferred reagent for controlling Clania variegate larva. The medicament should be applied during JulySeptember， namely before 2-3 years old of Clania vaiegate larva.

Key wordsClania variegate Snellen； Organic blueberry plantation； Toxicity determination； Field efficacy

大窠蓑蛾（Clania variegate Snellen）又叫大蓑蛾、大袋蛾，属鳞翅目蓑蛾科害虫，是近年来黔东南州有机蓝莓园的一种重要害虫。幼虫俗称“洞里毛”、“袋袋虫”、“吊死鬼”等，其食性杂，危害严重，幼虫体外有植物残屑和丝织成的袋囊，幼虫终生负囊生活，蚕食叶片造成大孔洞和缺刻，严重时叶片被取食殆尽[1-3]。大窠蓑蛾幼虫已严重危害贵州省黔東南州有机蓝莓产业的健康发展。

有机蓝莓是在“有机农业”规定下生产，拒绝任何人工合成的农药和化肥，生态控制自然承担了蓝莓有机生产过程中有害生物防治主要措施，并综合运用生物、物理等措施，对有害生物进行科学控制。随着蓝……