强俊叶 翟瑶 张静 等

[摘要] 目的 探討外源性硫化氢（H2S）对足月妊娠产妇离体子宫平滑肌收缩能力的影响情况。 方法 选取2014年6月～2015年5月河北省保定市第一医院56例足月已临产产妇（临产组）及56例未临产足月妊娠产妇（未临产组）的离体子宫平滑肌作为研究对象，分别浸浴于10-7、10-5、10-3 mol/L浓度的硫氢化钠（NaHS）溶液，观察两组肌条收缩的强度、收缩持续的时间以及收缩的频率；同时比较两组在不同浓度NaHS溶液浸浴时子宫肌条的收缩曲线下面积（AUC）抑制率与收缩频率抑制率。 结果 10-7、10-5、10-3 mol/L浓度NaHS溶液对临产组子宫平滑肌收缩AUC抑制率比较差异均有统计学意义（P < 0.05）。10-7、10-5、10-3 mol/L浓度NaHS溶液对未临产子宫平滑肌收缩AUC抑制率比较差异无统计学意义（P > 0.05），相同浓度NaHS溶液对临产组子宫平滑肌收缩AUC抑制率高于未临产组，差异均有统计学意义（P < 0.05）。10-7、10-5、10-3 mol/L浓度NaHS溶液对临产组子宫平滑肌收缩频率抑制率比较，差异均有统计学意义（P < 0.05），且随着NaHS溶液浓度的增高，抑制率逐渐增高。10-7、10-5、10-3 mol/L浓度NaHS溶液对未临产子宫平滑肌收缩频率抑制率比较差异无统计学意义（P > 0.05），临产组及未临产组在相同浓度NaHS溶液对子宫平滑肌收缩频率抑制率存在差异，临产组均高于未临产组（P < 0.05）。 结论 H2S对足月妊娠、已临产、离体子宫平滑肌收缩功能具有一定抑制作用，且该抑制作用随剂量增加而增加，提示其可能在分娩时宫收缩及舒张功能中发挥重要作用。

[关键词] 硫化氢；足月妊娠；子宫平滑肌；收缩功能

[中图分类号] R737.3 [文献标识码] A [文章编号] 1673-7210（2016）02（b）-0095-04

Influence of ectogenic hydrogen sulfide on the contraction of uterine smooth muscle in term pregnancy

QIANG Junye ZHAI Yao▲ ZHANG Jing ZHANG Shanshan WANG Qian LIU Yanyun MI Yilu

Department of Obstetrical， the First Hospital of Baoding City， Hebei Province， Baoding 071000， China

[Abstract] Objective To discuss the influence of ectogenic hydrogen sulfide on the contraction of excised uterine smooth muscle in term pregnancy. Methods Excised uterine smooth muscle of 56 full term pregnancy not in labor （non-labor group） and 56 full term pregnancy in labor （labor group） from June 2014 to May 2015 in the First hospital of Baoding City were selected as the research objects， and they were immersion bathed in 10-7， 10-5 mol/L and 10-3mol/L NaHS， and inhibition rate of contraction intensity duration， contraction frequency of the muscles in different concentrations of NaHS were observed； inhibition rate of AUC and contraction frequency of uterine muscle were compared in different concentrations of NaHS between two groups. Results There were statistical differencs in inhibition rate of AUC of uterine muscle in labor group among 10-7， 10-5 mol/L and 10-3 mol/L NaHS （P < 0.05）； there was no statistical difference in inhibition rate of AUC of uterine muscle in labor group among 10-7， 10-5 mol/L and 10-3 mol/L NaHS （P > 0.05）； there was statistical difference in inhibition rate of AUC of uterine muscle between two groups in same concentration of NaHS， and inhibition rate of AUC in labor group were higher than that in the non-labor group （P < 0.05）. There were statistical differences in inhibition rate of contraction frequency of uterine muscle in the labor group among 10-7， 10-5 mol/L and 10-3 mol/L NaHS （P < 0.05）， and with the increased of NaHS concentration， the inhibition rate was increased； there was no statistical difference in inhibition rate of contraction frequency of uterine muscle in labor group among 10-7， 10-5 mol/L and 10-3 mol/L NaHS （P > 0.05）； there were statistical differences in inhibition rate of contraction frequency of uterine muscle between two groups in same concentration of NaHS， and inhibition rate of contraction frequency in labor group was higher than that in non-labor group （P < 0.05）. Conclusion H2S has certain inhibition effect on contraction function of uterine smooth muscle in puerperal of parturients with full-term and in labor， and the inhibition increased with the increasing of the H2S dose， that prompt H2S may play an important role in contraction and relaxation function of uterine possible in the process of childbirth.

[Key words] Hydrogen sulfide； Term pregnancy； Uterine smooth muscle； Contractile function

硫化氢（H2S）气体是一种毒性气体，近年研究发现，H2S与NO、CO类似，均为人体内的气体信号分子[1]，具有重要的生理、生物学活性[2-3]。具有引起血管、消化道平滑肌舒张等作用，参与多个器官及系统生理功能的维持。有研究显示[4-5]，内源性H2S对子宫平滑肌收缩具有抑制功能，能够对子宫收缩及舒张功进行调节，提示其在维持子宫的生理功能中具有一定的作用，为了探讨H2S在足月妊娠子宫平滑肌收缩功能及其在分娩过程中的作用，本研究就外源性H2S对离体子宫平滑肌收缩能力的影响进行研究。……