刘晏 魏刚 陆兔林

[摘要] 目的 观察新痹痛灵对CIA大鼠脾脏T细胞亚群、白介素-2（IL-2）及白介素-2受体（IL-2R）的影响，探讨新痹痛灵治疗类风湿关节炎的作用机制。 方法 44只SD大鼠建立胶原诱导的关节炎模型（CIA），造模成功的36只大鼠随机分为4组： 模型组、阳性药组、新痹痛灵中剂量组、新痹痛灵高剂量组，每组各9只，另取9只正常SD大鼠为正常组。造模成功后连续给药28 d，末次给药后取血，运用流式细胞仪检测大鼠脾脏CD4+ T细胞及CD8+ T细胞的变化，ELISA检测观察大鼠脾脏IL-2、IL-2R。 结果 与模型组比较，新痹痛灵中剂量组、新痹痛灵高剂量组的CD4+/CD8+比值下调，IL-2与IL-2R水平下降，差异有统计学意义（P < 0.01或P < 0.05）。形态学观察显示：新痹痛灵对滑膜细胞增殖有抑制作用。 结论 新痹痛灵对T细胞亚群及其相关炎症因子的紊乱调节作用，可能是其治疗类风湿关节炎的机制之一。

[关键词] 新痹痛灵；CIA大鼠；T细胞亚群；IL-2；IL-2R

[中图分类号] R593 [文献标识码] A [文章编号] 1673-7210（2016）02（b）-0008-04

Effects of New Bitongling Compound on the T cell semi-group of CIA rats

LIU Yan1 WEI Gang2 LU Tulin3 GUO Haiying1 WANG Yue1

1.Nanjing University of Chinese Medicine， Jiangsu Province， Nanjing 210000， China； 2.Jiangsu Province Hospital of TCM， Jiangsu Province， Nanjing 210000， China；3.Nanjing University of Chinese Medicine， Jiangsu Province， Nanjing 210000， China

[Abstract] Objective To observe the effects of New Bitongling Compound on the T cell semi-group， IL-2 and IL-2R， and study the mechanism of New Bitongling Compound treating RA. Methods 44 SD rats were established collagen-induced arthritis （CIA） model， successful CIA 36 rats were randomly divided into 4 groups： model group， positive drug group， middle-dose New Bitongling Compound group， high-dose new Bitongling Compound group， with 9 rats in each group， 9 normal SD rats were as normal group. After model successfully established， the rats were given drugs for 28 d， blood sample was taken after the last administration. The CD4+ and CD8+ T cell in spleen of CIA rats were detected by flow cytometer， the IL-2 and IL-2R were detected by ELISA. Results Compared with model group， the CD4+/CD8+ ratio of middle-dose New Bitongling Compound group and high-dose New Bitongling Compound group decreased， the level of IL-2 and IL-2R also reduced， the differences were statistically significant （P < 0.01 or P < 0.05）. Morphological observation indicated that New Bitongling Compound had significant in-hibitory on synovial cells proliferation. Conclusion New Bitongling Compound can regulate the abnormal T cell semi-group IL-2 and IL-2R， which may be one of its mechanism of treatment of rheumatoid arthritis.

[Key words] New Bitongling Compound； CIA rats； T cell semi-group； IL-2；IL-2R

類风湿关节炎（RA）是一种以对称性多关节炎为主要表现的系统性、自身免疫性疾病，其基本病理改变是滑膜炎，病因研究至今尚无明确定论。研究表明，T细胞是RA关节滑膜中数量最多的一类炎症细胞，且处于激活状态，对RA病理的持续进展起重要作用[1]，而白介素2（IL-2）与白介素-2受体（IL-2R）是与T细胞密切相关的炎症因子，是反映机体细胞免疫功能的重要指标[2，3]。

新痹痛灵是从全国名老中医汪履秋教授治疗中医“痹证”（尤其是类风湿关节炎）的经验方变化而来，由朱丹溪上中下通用痛风方化裁，在结合了前期的实验及临床研究后总结而出。为进一步研究新痹痛灵治疗痹证的疗效和机制，本实验分别从T细胞亚群和炎症因子两个方面，对新痹痛灵进行了实验研究[4]。……