李美阳 金江山

摘要：为了研究不同培养条件对缬草（Valeriana fauriei var.dasycarpa Hara）不定根生长及其有效成分含量的影响，利用组织培养技术结合高效液相色谱，通过改变培养基蔗糖、氮源和磷源浓度，研究其对缬草不定根的生长和缬草三酯类、缬草烯酸类合成的影响。结果表明，当蔗糖浓度为60 g/L时最适合缬草不定根的生长以及总缬草三酯类和总缬草烯酸类的生产。NO3-/NH4+为1∶1时，不定根生长量和缬草三酯类以及缬草烯酸类达到最高。改变培养基NaH2PO4浓度时，缬草不定根生长均比不添加NaH2PO4时旺盛，但高浓度的NaH2PO4抑制了缬草三酯类和缬草烯酸类的合成，NaH2PO4为基本培养基的0.3倍时，不定根生长量最高，达14.89 g/L，增长率也最高，达到16.18倍，缬草烯酸类也达到最高产量为13.88 mg/L。

关键词：缬草（Valeriana fauriei var.dasycarpa Hara）；生物反应器；缬草烯酸：缬草三酯；蔗糖浓度；氮源；磷源

中图分类号：Q813.1+2 文献标识码：A 文章编号：0439-8114（2016）05-1304-03

DOI：10.14088/j.cnki.issn0439-8114.2016.05.055

Study on the Adventitious Roots Tissue Culture of Valeriana fauriei var. dasycarpa Hara

LI Mei-yang， JIN Jiang-shan

（College of Agriculture， Yanbian University， Yanji 133002， Jilin，China）

Abstract：To explore the effects of different culture conditions on Valeriana fauriei var. dasycarpa Hara adventitious roots growth and its effective components synthesis，the adventitious roots were obtained through tissue culture by manipulation of sucrose concentration， nitrogen and phosphate sources and the growth of plant， the content of valepotriates and valerenic acids were determined by HPLC. The results showed that when the sucrose concentration of 60 g/L were favorable for growth of adventitious roots and total valepotriates and valerenic acids production. The maximum root growth rates and content of valepotriates and valerenic acids were achieved while NO3-/NH4+ was 1∶1. To compare the media with adding NaH2PO4 and the medium without adding NaH2PO4，changing of NaH2PO4 concentration could favor for the adventitious root growth. But high NaH2PO4 concentration inhibited valepotriates and valerian acid biosynthesis. When NaH2PO4 in the basic culture medium of 0.3 times， growth of adventitious roots reached a maximum production of 14.89 g/L，growth rate was the highest， reached 16.18 times， total valerenic acids content was up to the highest yield 13.88 mg/L.

Key words：Valeriana fauriei var.dasycarpa Hara；bioreactor；valerenic acid；valepotriate；sucrose concentration；nitrogen sources；phosphate sources

纈草（Valeriana fauriei var.dasycarpa Hara）属于败酱科（Valerianaceae）缬草属（Valeriana）多年生草本植物，该属植物全世界约有250余种，主要分布于气候温和潮湿的地区， 遍布整个欧洲、亚洲北部、南美洲以及美国[1]。中国约有30种（含变种），主要分布于西南、西北及东北地区[2]。缬草在欧洲及美国常被用于治疗轻、重度失眠，具有镇静安神、解挛止痛、抗抑郁和抗肿瘤等作用，一直作为温和的镇静安神催眠药使用，缬草提取物及其制剂在国际上十分畅销，销售额列植物药前10位[3]。缬草的根和地下根茎作为镇静药已有很久的用药历史，中国早在明代李时珍的《本草纲目》中就有记载[4]，国外在Discorides时代也曾用过该药，古希腊人和古罗马人很早就认识到缬草具有镇静安神作用[5]。……