马怡 赵玉丹 江滔 杨孝容

摘要：用气相色谱-质谱联用技术鉴定马甲子果脂溶性成分，马甲子果用95%乙醇提取，浓缩液分别用石油醚、乙酸乙酯和正丁醇萃取，将石油醚层减压浓缩近干后用正己烷溶解，用气相色谱——质谱联用技术对其分析鉴定，以峰面积归一化法计算各组分的相对含量。结果检出51种化学组分，鉴定出其中28种，含量占总量的84.71%，其中甾类物质和酯类物质分别为65.13% 和11.38%。甾体是马甲子果的主要成分。

关键词：气相色谱-质谱联用；马甲子[Paliurus ramosissimus （Lour.） Poir.]果；脂溶性成分，测定

中图分类号：R284.2 文献标识码：A 文章编号：0439-8114（2016）05-1281-03

DOI：10.14088/j.cnki.issn0439-8114.2016.05.049

Determination for Liposoluble Compounds from the Fruit of

Paliurus Ramosissimus by GC-MS

MA Yia，ZHAO Yu-dana，JIANG Taob，YANG Xiao-rongb

（Leshan Normal University， a. Applied Chemistry， College of Chemistry； b. College of Chemistry， Leshan 614004， Sichuan， China）

Abstract： To analyze the liposoluble compounds from the fruit of Paliurus ramosissimus by gas chromatograph - mass sepectrometer （GC-MS）. The fruit solution concentrated with 95% ethanol was extracted by petroleum ether， ethyl acetate and n-butyl alcohol successively. The petroleum ether layer was evaporated and dissolved with hexane， then the liposoluble compounds were separated and identified by GC-MS. The relative content of each component was identified by method of peak area normalization. The results showed that 51 chemical components were separated and 28 components were identified， accounting about 84.71% from petroleum ether extracts， in which sterides and esters were 65.13% and 11.38% respectively. The sterides were the most liposoluble components in the fruit of Paliurus ramosissimus.

Key words：GC-MS；fruit of Paliurus ramosissimus；liposoluble components

马甲子为鼠李科铜钱树属植物马甲子[Paliurus ramosissimus （Lour.） Poir.]，又名铁篱笆、竻子、雄虎刺、铁菱角等，分布于西南及江苏、浙江、福建、江西、湖南、广西、台湾等地[1]。农户种植马甲子作果园、菜园、苗圃和鱼塘等围园篱笆卫士。民间马甲子是一种中药，主要功效为祛风散瘀，解毒消肿，用于治疗风湿痹痛，跌打损伤，咽喉肿痛，痈疽，除寒活血，发表解毒，疯狗咬伤等；马甲子果主治化瘀生新，治吐血，疗痔疮等，民间用于治疗不明肿块[1]。马甲子化学成分的研究主要集中在根和茎，有三萜类、环肽生物碱等[1-7]，马甲子果化学成分的研究较少，其成分有三萜类、香豆素类和二氢黄酮类等[8，9]。气相色谱-质谱联用（GC-MS）作为一种分析手段在挥发性组分和脂溶性成分测定方面应用广泛[10-15]，但将其用于定性分析马甲子果脂溶性成分的研究鲜见报道，本研究采用GC-MS定性测定马甲子果脂溶性组分，为马甲子果的开发利用提供参考。……