闫春生 牛美兰 田恒运

摘要：研究了馬齿苋口服液对高血脂大鼠瘦素（Leptin，LEP）、脂联素（Adiponectin，ADPN）、总胆固醇（Total triglyceride，TC）、总甘油三酯（Total triglyceride，TG）的影响。选取健康雄性SD大鼠65只， 随机分为模型对照组（15只）和正常对照组及马齿苋口服液低、中、高剂量组和罗格列酮组（每组10只）；正常对照组大鼠饲喂基础饲料，模型对照组大鼠饲喂高脂饲料，其余组大鼠饲喂高脂饲料，并进行药物干扰，喂养12周后，测定大鼠血清瘦素、脂联素、TC、TG的含量。结果表明，①瘦素：与正常对照组相比，罗格列酮组显著升高，模型组、马齿苋水煎剂低、中剂量组瘦素极显著升高，高剂量组升高不显著；与模型对照组比较，其余组均极显著降低；与低剂量组比较，中剂量组显著降低，正常组、高剂量组均极显著降低；马齿苋水煎剂高剂量组与罗格列酮组血清瘦素差异不显著。②脂联素：与正常对照组相比，其余组脂联素含量极显著降低；与模型组比较，其余组脂联素含量均极显著升高；马齿苋水煎剂低剂量与中剂量组差异不显著，低剂量组显著低于高剂量组，马齿苋水煎剂高剂量组与罗格列酮组差异不显著。③TC、TG含量表现一致，均为马齿苋口服液低剂量组、模型对照组极显著高于其余组；高剂量组显著低于中剂量组；正常对照组、高剂量组、罗格列酮组间差异不显著。马齿苋口服液可能是通过提高大鼠血清中脂联素，降低瘦素、TC、TG含量来预防大鼠高血脂的。

关键词：瘦素；脂联素；马齿苋；脂代谢

中图分类号：R589.2 文献标识码：A 文章编号：0439-8114（2016）05-1221-03

DOI：10.14088/j.cnki.issn0439-8114.2016.05.033

Effect of Purslane Oral Liquid on Lipid Metabolism in Hyperlipidemia Rats

YAN Chun-sheng，NIU Mei-lan，TIAN Heng-yun

（Huanghe Science and Technology College，Zhengzhou 450063，China）

Abstract： In this experiment， 65 healthy male SD rats were randomly divided into 6 groups， such as model group， normal control group，purslane oral liquid of low， middle and high dose group and rosiglitazone group， to analyze the effects of purslane oral liquid on the level of serum leptin （LEP）， adiponectin （ADPN），total cholesterol （TC）， total triglyceride （TG） in hyperlipidemia rats. The rats in normal control group were fed with basal diet，while the model group rats were fed high fat diet， the other group rats were given high fat diet and drug for 12 weeks.The contents of LEP and ADPN were detected by enzyme linked immunosorbent assay （ELISA） and the content of TC and TG were detected by automatic biochemical analyzer. The results showed that the leptin in rosiglitazone group significantly increased and extreme significantly increased in model group， low and middle purslane oral liquid group， while no significant changed in high dose of purslane oral liquid group when compared with normal control group. But when compared with the model control group， the LEP content was significantly decreased in the other groups. When compared with the low dose of purslane oral liquid group， the LEP content in middle dose was group significantly lower， and in normal control group and high dose group were extreme significantly lower. There had no significant difference in LEP content between high dose of purslane oral liquid group and rosiglitazone group. Compared with normal control group， the ADPN content in other groups were extreme significantly lower but when compared with model control group， the ADPN contents in other groups were extreme significantly higher. There had no significant difference in ADPN content between the low and middle dose of purslane oral liquid， and the high dose of purslane oral liquid and rosiglitazone group. But the ADPN content in low dose of purslane oral liquid was significantly lower than that in high dose group. The contents of TC and TG in low dose of purslane oral liquid group and model group were extreme significantly higher than other groups. The contents in middle dose group was significantly lower than those in low dose group. There had no significant difference in normal control group，high dose group and rosiglitazone group. It indicated that the purslane oral liquid might have effect of hyperlipidemia prevention through increasing the content of adiponectin and decreasing leptin levels and the content of TC，TG in serum.

Key words：leptin；adiponectin；purslane；lipid metabolism

1 材料与方法