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辽河保护区五种委陵菜在PEG胁迫下渗透调节物质可溶性蛋白质与脯氨酸变化

2016-10-19李楠孙航张姝哲陈颖刘淼曲波

湖北农业科学 2016年8期

李楠 孙航 张姝哲 陈颖 刘淼 曲波

摘要:采用聚乙二醇模拟干旱胁迫,研究5种委陵菜(Potentilla)抗旱性,探索干旱脅迫对5种委陵菜属植物渗透调节物质可溶性蛋白质与脯氨酸含量的影响。结果表明,干旱胁迫处理后委陵菜可溶性蛋白相对含量有所增加,干旱胁迫1~5 d莓叶委陵菜可溶性蛋白相对含量增幅最大,处理7 d蛇含委陵菜可溶性蛋白质相对含量增幅最大,而蔓委陵菜与三叶委陵菜可溶性蛋白质相对含量增幅较小,且在20%PEG处理第5天后下降。干旱胁迫处理使5种委陵菜叶片脯氨酸相对含量上升,其中蛇含委陵菜与莓叶委陵菜脯氨酸相对含量增幅最大;10%PEG与15%PEG胁迫处理后5种委陵菜脯氨酸相对含量均呈上升趋势,20%PEG胁迫处理第5天伏委陵菜与三叶委陵菜叶片中的脯氨酸相对含量即呈现下降趋势。5种委陵菜抗旱能力依次为蛇含委陵菜>莓叶委陵菜>蔓委陵菜>伏委陵菜>三叶委陵菜。

关键词:委陵菜(Potentilla);干旱胁迫;可溶性蛋白质;脯氨酸

中图分类号:Q945 文献标识码:A 文章编号:0439-8114(2016)08-1936-03

DOI:10.14088/j.cnki.issn0439-8114.2016.08.009

Abstract: In this paper, utilizing polyethylene glycol to simulate drought stress,drought resistance was studied on 5 species of Potentilla. The influence of drought stress on the content of soluble protein and proline was also studied.Under the drought stress,soluble protein content of 5 species of Potentilla increased. From 1 day to 5 days, the soluble protein content of Potentilla fragarioides shows the largest increase.In 7 days, the protein content of Potentilla kleiniana increased most. in the soluble protein content of Potentilla flagellaris and Potentilla freyniana increased less, which decreased in 5 d after treated by 20% PEP. Under drought stress,proline content of 5 Potentilla increased.In Potentilla kleiniana and Potentilla fragarioides,the proline content increased most.Also,the proline content of 5 species of Potentilla increased after treated by 10% and 15% PEG. Treated by 20% PEG,the relative content of proline in Potentilla paradoxa and Potentilla freyniana decreased after 5 days. The drought resistance of the five Potentilla is gauged as follows: Potentilla kleiniana, Potentilla fragarioides, Potentilla flagellaris, Potentilla paradoxa, Potentilla freyniana.

Key words: Potentilla;drought stress;soluble protein;proline

辽河是中国七大江河之一,发源于河北省七老图山脉之光头山,流经河北、内蒙古、吉林、辽宁,至盘锦注入渤海,流域面积 21.96×104 km2,全长 1 345 km。由于长期开垦耕作,导致河岸带沙化、盐碱化,水土流失、风沙侵蚀日益严重,生态环境脆弱,已经成为制约其生态环境恢复、生态产业发展的重要因素之一[1]。辽河保护区是2010年3月经中国共产党辽宁省委员会、辽宁省人民政府批准,依辽河干流设立的狭长区域。辽河保护区始于东西辽河交汇处(铁岭福德店),穿越铁岭、沈阳、鞍山、盘锦,终于盘锦入海口。辽河保护……

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