木林洁程锦国△姜程曦（.温州医科大学，浙江 温州 325000；2.浙江中医药大学附属温州市中医院，浙江 温州325000）

肾病一号方总皂苷对肾纤维化大鼠的治疗作用*

木林洁1，2程锦国1，2△姜程曦1
（1.温州医科大学，浙江 温州 325000；2.浙江中医药大学附属温州市中医院，浙江 温州325000）

目的 观察肾病一号方及其总皂苷对肾纤维化大鼠的治疗作用。方法 雄性SD大鼠30只，随机分为假手术组、UUO模型组、洛丁新组、肾病一号方组、总皂苷组。采用单侧输尿管结扎制作UUO大鼠模型，造模14 d后收集24 h尿液，采用双缩脲法测定24 h尿蛋白含量（24 h UP）。处死大鼠取血清检测尿素氮（BUN）和血肌酐（Scr），取肾组织行HE染色评价肾小管间质纤维化损伤程度，免疫组化方法观察转化生长因子-β1 （TGF-β1）的表达情况。结果 肾病一号方组和总皂苷组大鼠肾纤维化明显改善，TGF-β1表达水平降低，与模型组差异有统计学意义（P＜0.05）。结论 肾病一号方及其总皂苷能明显改善肾纤维化，其作用机制可能是通过下调TGF-β1的表达，从而达到肾脏保护的作用。肾病一号方总皂苷可能是肾病一号方防治肾纤维化的药效学物质基础之一。

肾纤维化 单侧输尿管结扎 转化生长因子-β1

【Abstract】Objective：To observe the therapeutic effect of Nephropathy 1st decoction and saponin in renal fibrosis rats.Methods：30 male SD rats were randomly divided into the sham operation group，UUO model group，Lotensin group，Nephropathy 1st decoction group and saponin group.A rat model of renal fibrosis was induced by unilateral ureteral ligation.Rats were executed after 14 days.24 h urinary protein（24h UP），serum blood urea nitrogen（BUN）and serum creatitine（Scr）were tested.Renal tubular interstitial fibrosis was evaluated by Hematoxylin and eosin staining（HE）.The expression of transforming growth factor-beta 1（TGF-β1）was observed with immunohistochemical method.Results：The improvement of renal fibrosis in Nephropathy 1st decoction group and saponin group was obvious；the expression level of TGF-β1 decreased and compared with the model group，there were significant differences（P＜0.05）.Conclusion：Nephropathy 1st decoction and saponin may ameliorate renal interstitial fibrosis by down-regulating the expression levels of TGF-β1.Saponin may be one of the pharmacodynamic material bases of Nephropathy 1st decoction.

【Key words】Renal fibrosis；Unilateral ureteral obstruction；Transforming growth factor-beta 1

随着我国人口老龄化以及糖尿病、高血压患病人数的增加，慢性肾脏病的发病率正在逐年上升［1］，给家庭和社会带来了巨大压力。慢性肾脏病在人类死亡的主要原因中仍然居于第5位至9位之间。因此，慢性肾功能衰竭仍然是人类生存的重要威胁之一［2］。研究表明肾纤维化是各种慢性肾脏疾病进展至终末期的共同通路［3］。……