白志超陈名超李鹤飞（.河北省邯郸市中心医院，河北 邯郸 05600；.河北工程大学附属医院，河北 邯郸05600）

银杏内酯B对心肌缺血再灌注损伤大鼠氧化应激反应的影响*

白志超1△陈名超2李鹤飞1
（1.河北省邯郸市中心医院，河北 邯郸 056001；2.河北工程大学附属医院，河北 邯郸056002）

目的 观察银杏内酯B（GB）对心肌缺血再灌注损伤大鼠氧化应激反应的影响并探讨其机制。方法将120只大鼠按照随机数字表法分为假手术组，模型组，GB高、中、低剂量组和地尔硫组，每组20只；采用夹闭冠状动脉的方法制备心肌缺血再灌注损伤大鼠模型。TTC染色法计算心肌梗死面积，生化分析法检测心肌组织中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）活性和丙二醛（MDA）含量，血清中谷草转氨酶（AST）、磷酸肌酸激酶（CPK）、乳酸脱氢酶（LDH）活性，HE染色法观察心肌组织形态结构改变，TUNEL法观察心肌细胞凋亡状况并计算凋亡指数 （AI）；Western blot法检测心肌组织中NF-κB蛋白表达并进行半定量分析。结果 与模型组比较，GB高中低剂量组大鼠心肌组织梗死面积显著降低，SOD、GSH-Px、CAT活性显著升高且MDA含量显著降低，血清中AST、CPK、LDH活性显著降低 （P＜0.05或P＜0.01）；GB各组心肌组织病变和心肌细胞凋亡状况较模型组均呈不同程度改善，其中30、60 mg/kg组AI显著降低，NF-κB蛋白表达显著下调（P＜0.05或P＜0.01）。结论 GB具有抑制心肌缺血再灌注损伤大鼠氧化应激反应的作用，这一作用可能与其能够降低心肌缺血再灌注后心肌损伤有关。

银杏内酯B 心肌 缺血再灌注 氧化应激 影响

【Abstract】Objective：To investigate the effects and mechanism of Ginkgolide B（GB）on oxidative stress in rats with myocardial ischemia-reperfusion injury.Methods：120 rats were randomly divided into six groups：the sham group，the model group，GB high/middle/low groups and diltiazem group，20 in each.The models with myocardial ischemia-reperfusion injury were made by clamping the artery.The areas of myocardial infarction was analyzed by TTC staining；the activity of SOD，GSH-Px，CAT and the content of MDA in myocardial tissue were determined，as well as the activity of AST，CPK，LDH in serum；the histopathological changes of myocardial tissue was observed by HE staining；the apoptosis of cardiomyocytes was observed by TUNEL and the apoptosis index（AI）was calculated；the expression of NF-κB was examined by Western blotting and semi-quantitative analysis.Results：Compared with the model group，the myocardial infarction areas in GB（30，60 mg/kg）groups were significantly decreased；the activity of SOD，GSH-Px and CAT were significantly increased and the activity of MDA was significantly decreased；the level of AST，CPK and LDH in serum were significantly decreased；the myocardial tissue histopathological changes and cardiomyocytes apoptosis were significantly improved；AI in GB （30，60 mg/kg）groups were significantly decreased；the expression of NF-κB was significantly decreased.All of the difference above were significant（P＜0.05 or P＜0.01）.Conclusion：GB has inhibitive effects on oxidative stress in the rats with myocardial ischemia-reperfusion injury，which is perhaps related to its effects on reducing myocardial injury after myocardial ischemia-reperfusion.

【Key words】GB；Myocardial；Ischemia-reperfusion；Oxidative stress；Effects

近年来，急性心肌梗死的发病率逐年升高，已经逐渐发展成为危害人类生命健康的主要疾病之一。……